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Engineered protein G variants for multifunctional antibody-based assemblies.
Protein Sci
February 2025
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from multiple pairs of native and modified Fab scaffolds and protein G (PG) variants engineered by phage display to introduce high pair-wise specificity. The variety of possible Fab-PG pairings provides a highly orthogonal system that can be exploited to perform challenging cell biology operations in a straightforward manner.
View Article and Find Full Text PDFRelax: Analytic and automatic NMR relaxation theory.
J Magn Reson
January 2025
NMR Research Unit, University of Oulu, P.O. Box 3000, FI-90014, Finland. Electronic address:
Spin relaxation is modelled using the so-called relaxation superoperator Γˆˆ. Analytic forms of Γˆˆ have been derived in the literature in the simplest cases of one- or two-spin systems, with S=12 nuclei and no more than two different simultaneous relaxation mechanisms involved. Beyond that, for systems of more than two spins, with S>12 and/or multiple relaxation mechanisms at play, the derivations become notoriously complicated, which is why analytic relaxation theory has mostly been considered a dead end.
View Article and Find Full Text PDFHighly Elastic Spongelike Hydrogels for Impedance-Based Multimodal Sensing.
ACS Nano
January 2025
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Republic of Singapore.
Hydrogel-based sensors have been widely studied for perceiving the environment. However, the simplest type of resistive sensors still lacks sensitivity to localized strain and other extractable data. Enhancing their sensitivity and expanding their functionality to perceive multiple stimuli simultaneously are highly beneficial yet require optimal material design and proper testing methods.
View Article and Find Full Text PDFImpact of increasingly complex cell culture conditions on the proteome of human periodontal ligament stem cells.
Regen Med
January 2025
Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country (UPV/EHU), Leioa, Spain.
Aims: Human periodontal ligament stem cells (hPDLSCs) exhibit an enormous potential to regenerate periodontal tissue. However, their translatability to the clinical setting is constrained by technical difficulties in standardizing culture conditions. The aim was to assess complex culture conditions using a proteomic-based protocol to standardize multi-layer hPDLSC cultivation methodology.
View Article and Find Full Text PDFPolarization-resolved transmission matrices of specialty optical fibers.
Rev Sci Instrum
December 2024
OFS Laboratories, 19 Schoolhouse Road, Somerset, New Jersey 08873, USA.
Transmission matrix measurements of multimode fibers are now routinely performed in numerous laboratories, enabling control of the electric field at the distal end of the fiber and paving the way for the potential application to ultrathin medical endoscopes with high resolution. The same concepts are applicable to other areas, such as space division multiplexing, targeted power delivery, fiber laser performance, and the general study of the mode coupling properties of the fiber. However, the process of building an experimental setup and developing the supporting code to measure the fiber's transmission matrix remains challenging and time consuming, with full details on experimental design, data collection, and supporting algorithms spread over multiple papers or lacking in detail.
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