Effect of dexamethazone on hepatic vascular response in experimental sepsis.

Background: The aim of the present study was to evaluate the effect of dexamethasone-pretreatment on the hepatic artery and portal vein of septic rats, which were generated by lipopolisaccarides (LPS) intraperitoneal injection.

Method: Thirty-six albino Wistar rats were used and constructed as LPS (n = 12), control (n = 12), dexamethazone-pretreatment (n = 6) and dexamethazone-control (n = 6) groups. Hepatic artery and portal vein rings were excised and placed in Krebs-Henseleit solution. Vessel rings were contracted with phenylephrine adding to the organ chamber in cumulative doses. Then the contraction-response curves were drawn.

Results: In the LPS group, phenylephrine evoked contractions were reduced in both hepatic artery and portal vein rings in comparison to the control group. In the dexamethasone-control group, phenylephrine-evoked contractions were increased but not significantly. Dexamethasone-pretreatment increased the phenylephrine-evoked contractions close to the values of control group for both types of rings.

Conclusions: Dexamethasone pretreatment corrected the vascular hyporeactivity to phenyleprine in isolated portal vein and hepatic artery rings prepared from the LPS treated rats in experimental sepsis. This might have occurred as a result of inhibition of inducible nitric oxide synthase expression.