Fifteen countries, based on geographical representation, Interbull membership, and size of progeny testing programs, provided a brief description of national selection index and top bull listings from August 2003. Individual traits included in each selection index were grouped into 3 components as they related to production, durability, and health and reproduction. The relative emphasis for each component within the selection index, as well as the number of common bulls among top listings were compared across countries. Average relative emphasis for production, durability, and health and reproduction, across all countries, was 59.5, 28, and 12.5%, respectively. The main difference between selection indices in various countries was the relative emphasis on production. Overall, the Danish S-Index had the most balanced emphasis across the 3 components, with 34% on production, 29% on durability, and 37% on health and reproduction. Broadening of breeding goals through recent changes to selection indices decreased the similarities of top bull listings across the various countries, with a slightly greater commonality among sires of top bulls.
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http://dx.doi.org/10.3168/jds.S0022-0302(05)72792-2 | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611.
We describe a microfluidic device to extract DNA from a cell lysate, without the need for centrifuges, magnetic beads, or gels. Instead, separation is driven by transverse migration of DNA, which occurs when a polyelectrolyte solution flowing through a microfluidic channel is subjected to an electric field. The coupling of the weak shearing with the axial electric field is highly selective for long, flexible, charged molecules, of which DNA is the sole example in a typical cell lysate.
View Article and Find Full Text PDFNeurosurg Rev
January 2025
Department of Neurosurgery, Hospital Universitario Fundación Jiménez Díaz, Av. De los Reyes Católicos, 2, Madrid, 28040, Spain.
Matched-controlled long-term disease evaluation and neuropsychological outcomes derived from deep brain stimulation of the subthalamic nucleus (STN-DBS) in Parkinson´s disease (PD) are lacking, with inconsistent results regarding the cognitive impact of this procedure. Here we study the long-term effects associated to DBS comparing outcomes with a matched control group. A prospective observational study of 40 patients with PD with bilateral STN-DBS, with a mean follow-up of 9 (6-12) years was conducted.
View Article and Find Full Text PDFMol Divers
January 2025
Key Laboratory of Structure-Based Drugs Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang, PR China.
ROCK inhibitors can inhibit IL-1β and NLRP3, and their therapeutic potential for osteoarthritis and rheumatoid arthritis has been confirmed, but their impact on gouty arthritis has not been reported yet. By hybridization the structure of Edaravone, a series of ROCK inhibitors with pyrazolone scaffold were designed and synthesized. RM-04 has acceptable selective ROCK2 inhibitory activity with an IC of 4.
View Article and Find Full Text PDFMikrochim Acta
January 2025
Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, College of Optoelectronic Materials and Technology, Jianghan University, Wuhan, 430056, China.
An electrochemical sensor is presented for the detection of the chloramphenicol (CAP) based on a bimetallic MIL-101(Fe/Co) MOF electrocatalyst. The MIL-101(Fe/Co) was prepared by utilizing mixed-valence Fe (III) and Co (II) as metal nodes and terephthalic acid as ligands with a simple hydrothermal method and characterized by SEM, TEM, XRD, FTIR, and XPS. Electrochemical measurements such as electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) showed that bimetallic MIL-101(Fe/Co) had the faster electron transfer, larger electroactive area, and higher electrocatalytic activity compared with their monometallic counterparts due to the strong synergistic effect between bimetals.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
January 2025
Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland.
Metagenomes present a source for novel enzymes, but under 1% of environmental microbes are cultivatable. Because of its useful properties, Escherichia coli has been used as a host organism in functional genomic screens. However, due to differing expression machineries in the expression host compared to the source organism of the DNA sequences, screening outcomes can be biased.
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