The Cre/loxP recombination system of bacteriophage P1 is one of the most powerful tools in genome engineering. We report, however, that the activity of the Cre/loxP system interferes with the stability of the multicopy loxP-bearing plasmids in Escherichia coli recA bacteria. Due to the predominantly unidirectional Cre-mediated high-order multimer formation of these plasmids, the number of their copies (overall yield) gradually decreases. Intermolecular recombination reduces the copy number of plasmids and eventually increases their segregational instability. We have found that in the presence of even the slightest amount of Cre activity, loxP-bearing plasmids continuously undergo multimerization, which very rapidly leads to loxP-plasmid free cells. Our results are compatible with the hypothesis of the multimer catastrophe [Cell, 1984 (36), 1097].
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.plasmid.2004.04.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rspo3-mediated metabolic liver zonation regulates systemic glucose metabolism and body mass in mice.
PLoS Biol
January 2025
Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
The unique architecture of the liver consists of hepatic lobules, dividing the hepatic features of metabolism into 2 distinct zones, namely the pericentral and periportal zones, the spatial characteristics of which are broadly defined as metabolic zonation. R-spondin3 (Rspo3), a bioactive protein promoting the Wnt signaling pathway, regulates metabolic features especially around hepatic central veins. However, the functional impact of hepatic metabolic zonation, regulated by the Rspo3/Wnt signaling pathway, on whole-body metabolism homeostasis remains poorly understood.
View Article and Find Full Text PDFCombining Cre-LoxP and single-cell sequencing technologies: insights into the extracellular vesicle cargo transfer.
Extracell Vesicles Circ Nucl Acids
November 2024
Animal Physiology and Immunology, School of Life Sciences, Technical University of Munich, Freising 85354, Germany.
The recent study from the Pogge von Strandmann group published in , by Alashkar Alhamwe ., combined for the first time the Cre-LoxP recombination system with single-cell sequencing. The group monitored the tumor-derived extracellular vesicle (EV) uptake and the EV functions in the recipient non-malignant cells in a pancreatic ductal adenocarcinoma mouse model.
View Article and Find Full Text PDFLyve1-Driven Nras Causes Edema, Enlarged Lymphatic Vessels, and Hepatic Vascular Defects in Embryonic Mice.
Pediatr Blood Cancer
March 2025
Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center and University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.
Background: Kaposiform lymphangiomatosis (KLA) is a complex lymphatic anomaly associated with a somatic activating NRAS p.Q61R (NRAS) mutation. KLA is characterized by malformed lymphatic vessels that can lead to effusions and coagulopathy.
View Article and Find Full Text PDFInclusive, exclusive and hierarchical atlas of NFATc1/PDGFR-α cells in dental and periodontal mesenchyme.
Elife
December 2024
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Platelet-derived growth factor receptor alpha (PDGFR-α) activity is crucial in the process of dental and periodontal mesenchyme regeneration facilitated by autologous platelet concentrates (APCs), such as platelet-rich fibrin (PRF), platelet-rich plasma (PRP) and concentrated growth factors (CGF), as well as by recombinant PDGF drugs. However, it is largely unclear about the physiological patterns and cellular fate determinations of PDGFR-α cells in the homeostasis maintaining of adult dental and periodontal mesenchyme. We previously identified NFATc1 expressing PDGFR-α cells as a subtype of skeletal stem cells (SSCs) in limb bone in mice, but their roles in dental and periodontal remain unexplored.
View Article and Find Full Text PDFNatural small molecule hinokitone mitigates NASH fibrosis by targeting regulation of FXR-mediated hepatocyte apoptosis.
J Adv Res
December 2024
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Haidian District, Beijing 100191, China; State Key Laboratory of Vascular Homeostasis and Remodeling, State Key Laboratory of Natural and Biomimetic Drugs, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Peking University, Beijing 100191, China; Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, Beijing 100191, China. Electronic address:
Introduction: Liver fibrosis is the common fate of NASH and poses a major health threat with very limited pharmacological treatments.
Objectives: This study aims to investigate the preventive effect of hinokitone (HO), an isolated compound from Agathis dammara, on NASH fibrosis and its underlying mechanism.
Methods: To investigate the effect of HO on NASH fibrosis, C57BL/6 mice were either fed a high-fat diet (HFD) in conjunction with intraperitoneal injection of CCl for 8 weeks or single CCl for 14 days to establish mouse liver fibrosis model, and HO was administered by gavage simultaneously.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!