AI Article Synopsis
- Polymerase chain reaction (PCR) was employed with random 10-mer primers to amplify genomic DNA in a cultivated alfalfa backcross population.
- Out of 19 tested primers, 13 successfully amplified 37 polymorphic fragments, with 28 of those (76%) showing dominant Mendelian segregation.
- The findings suggest that RAPD markers can be effectively utilized to develop genetic information in species like alfalfa, where existing knowledge is limited.
Article Abstract
Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/g92-014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!