This study was undertaken to investigate the effects of a deficit in protease inhibitor (AT) induced by intravenously administered trypsin on the development of elastase-induced emphysema. Rats receiving a perfusion of trypsin (4.5 mg/kg body wt) intravenously (TIV rats) or one instillation of elastase (92 IU/subject) into the trachea (ELAS rats) were compared with rats receiving both trypsin and elastase (TIVELAS rats). Compared with 8 sham-injected rats, the serum AT activity of 14 TIV rats decreased slightly (5.5%) 150 min after the beginning of the perfusion. In six other TIV rats sacrificed early after the perfusion, a granulocyte sequestration with edema and vascular thrombi demonstrated early lung injury. Anatomical studies of lung and determination of the mean linear intercept (MLI) were carried out 56 days after the administration of the enzymes. Emphysema was confirmed by a significant (P less than .001) MLI increase (about 150 microns) in 22/24 TIV, 20/21 ELAS, and 21/21 TIVELAS rats in comparison with 40 control rats (78 microns). These similar results of the treated rats show that trypsin did not worsen elastase-induced emphysema and also indicate that trypsin given intravenously alone induces emphysema as does elastase when introduced into the airways. The AT activity decrease consequent to proteolysis by trypsin and pulmonary leucostasis may contribute to this trypsin-triggered emphysema.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3109/01902149209020650 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Potential of the zebrafish (Danio rerio) embryo test to discriminate between chemicals of similar molecular structure-a study with valproic acid and 14 of its analogues.
Arch Toxicol
November 2022
Aquatic Ecology and Toxicology Group, Center for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 504, 69120, Heidelberg, Germany.
Valproic acid is a frequently used antiepileptic drug and known pediatric hepatotoxic agent. In search of pharmaceuticals with increased effectiveness and reduced toxicity, analogue chemicals came into focus. So far, toxicity and teratogenicity data of drugs and metabolites have usually been collected from mammalian model systems such as mice and rats.
View Article and Find Full Text PDFEvolution of Brain Morphology in Spontaneously Hypertensive and Wistar-Kyoto Rats From Early Adulthood to Aging: A Longitudinal Magnetic Resonance Imaging Study.
Front Aging Neurosci
November 2021
Department of Medical Imaging, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
The influence of hypertension and aging alone on brain structure has been described extensively. Our understanding of the interaction of hypertension with aging to brain morphology is still limited. We aimed to detect the synergistic effects of hypertension and aging on brain morphology and to describe the evolution patterns of cerebral atrophy from spatial and temporal perspectives.
View Article and Find Full Text PDFAntidepressant effects of total iridoids of via the intestinal flora-blood-brain barrier pathway.
Pharm Biol
December 2021
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, PR China.
Context: Jones [syn. DC, (Valerianaceae)] (VJJ) is used to treat depression.
Objective: To explore the effects of total iridoids of VJJ extract (TIV) on chronic unpredictable mild stress (CUMS) in mice.
A simple LC-MS/MS method facilitated by salting-out assisted liquid-liquid extraction to simultaneously determine trans-resveratrol and its glucuronide and sulfate conjugates in rat plasma and its application to pharmacokinetic assay.
Biomed Chromatogr
November 2017
Clinical Pharmacokinetics Research Laboratory, China Pharmaceutical University, Nanjing, Jiangsu, China.
A simple LC-MS/MS method facilitated by salting-out assisted liquid-liquid extraction (SALLE) was applied to simultaneously investigate the pharmacokinetics of trans-resveratrol (Res) and its major glucuronide and sulfate conjugates in rat plasma. Acetonitrile-methanol (80:20, v/v) and ammonium acetate (10 mol L ) were used as extractant and salting-out reagent to locate the target analytes in the supernatant after the aqueous and organic phase stratification, then the analytes were determined via gradient elution by LC-MS/MS in negative mode in a single run. The analytical method was validated with good selectivity, acceptable accuracy (>85%) and low variation of precision (<15%).
View Article and Find Full Text PDFImidazoline 1 receptor activation preserves respiratory drive in spontaneously breathing newborn rats during dexmedetomidine administration.
Paediatr Anaesth
May 2017
Department of Dental Anesthesiology, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan.
Background: Dexmedetomidine is an alpha-2 (α ) adrenoceptor and imidazoline 1 (I ) receptor agonist that provides sedation without loss of respiratory drive.
Aims: The aim of this study was to elucidate the involvement of α -adrenoceptor and I receptor in the cardiorespiratory changes induced by dexmedetomidine in spontaneously breathing newborn rats.
Methods: An abdominal catheter to administer drugs and three subcutaneous electrodes to record electrocardiographic data were inserted into 2- to 5-day-old Wistar rats under isoflurane anesthesia.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!