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Evaluation of the recombinant protein -TSP-2 for the serological diagnosis of imported urogenital schistosomiasis and comparison with commercially available tests.
Parasitology
January 2025
Tropical Medicine Unit, Hospital Universitario Poniente, Ctra. de Almerimar 31, 04700 El Ejido, Spain.
Different agencies have emphasized the need to evaluate current serological methods for screening patients with suspected urogenital schistosomiasis. However, there is still a lack of evidence regarding the most appropriate methods for this purpose. Here we assessed the diagnostic efficacy of a newly developed serological technique that utilizes the recombinant protein -TSP-2, applied to the urine and serum of migrants suspected of having urogenital schistosomiasis.
View Article and Find Full Text PDFEnhancing the Bothropic Antivenom through a Reverse Antivenomics Approach.
J Proteome Res
January 2025
Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04023-901, Brazil.
Antivenoms are the only effective treatment for snakebite envenomation and have saved countless lives over more than a century. Despite their value, antivenoms present risks of adverse reactions. Current formulations contain a fraction of nonspecific antibodies and serum proteins.
View Article and Find Full Text PDFDiagnostic methods and protocols for rapid determination of methicillin resistance in Staphylococcus aureus bloodstream infections: a comparative analysis.
Eur J Clin Microbiol Infect Dis
January 2025
Microbiology and Virology Unit, University Hospital Città Della Salute E Della Scienza Di Torino, Turin, Italy.
Purpose: To evaluate diagnostic performance of four diagnostic methods for rapid determination of methicillin resistance in S. aureus positive blood cultures (BCs).
Methods: Clinical and spiked BCs were subjected to the evaluation of the following methods and protocols: a.
Strategy to mitigate aggregation during Protein A chromatography and low pH virus inactivation for a nivolumab biosimilar candidate.
J Chromatogr A
January 2025
Center of Molecular Immunology, 216 Street and 15th Avenue Atabey-Siboney Playa P.O. Box 16040, Havana, 11600, Cuba. Electronic address:
Protein A chromatography represents the most prevalent methodology for the capture of monoclonal antibodies. The use of a low pH elution buffer from Protein A has been observed to contribute to product aggregation, particularly in the case of IgG4 antibodies, such as nivolumab. This paper presents a well-defined strategy for addressing this issue.
View Article and Find Full Text PDFEstablishment of DAS-ELISA and lateral flow immunochromatography for the detection of peanut allergen Ara h 3 after heat-moisture treatment.
Food Chem
January 2025
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China. Electronic address:
Peanuts are highly nutritious but pose a significant risk of triggering food allergies. While heat treatment can reduce the allergenicity of many foods, it may also alter their structure, potentially impacting detection results. This study employed double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and lateral flow immunochromatography (LFIA) to evaluate the allergen Ara h 3 following heat-moisture treatment.
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