AI Article Synopsis
- The ade6-M26 mutation in the yeast Schizosaccharomyces pombe creates a hotspot for meiotic recombination, primarily dependent on the heptamer sequence 5'-ATGACGT-3'.
- Researchers discovered that despite the heptamer's presence, some instances did not activate the hotspot, leading to the identification of an 18-bp consensus binding sequence for the transcription factor Atf1-Pcr1 that includes the heptamer.
- Mutations around the heptamer significantly enhanced hotspot activity, suggesting that M26 is a larger recombination hotspot than previously believed, which may offer insights into the mechanisms of meiotic recombination.
Article Abstract
The ade6-M26 mutation of Schizosaccharomyces pombe created a meiotic recombination hotspot. Previous analyses indicated that the heptamer 5'-ATGACGT-3' was necessary and sufficient for hotspot activity; the Atf1-Pcr1 transcription factor binds to this sequence and activates M26. After finding cases in which the M26 heptamer in ade6 was, surprisingly, not active as a hotspot, we used an in vitro selection method (SELEX) that revealed an 18-bp consensus sequence for Atf1-Pcr1 binding, 5'-GNVTATGACGTCATNBNC-3', containing the M26 heptamer at its core. Using this consensus sequence as a guide, we made mutations on each side of the heptamer at two separate sites in ade6. These mutations increased the intracellular hotspot activity of the heptamer, in some cases by >15-fold. These results show that M26, the eukaryotic recombination hotspot with the most precisely defined nucleotide sequence, is larger than previously thought, and they provide valuable information for clarifying the role of M26, and perhaps other hotspots, in meiotic recombination.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449614 | PMC |
| http://dx.doi.org/10.1534/genetics.104.039230 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic variation in Arabidopsis thaliana reveals the existence of natural heat resilience factors for meiosis.
Plant Physiol
December 2024
Arameiosis Lab, South-Central Minzu University, Wuhan 430074, China.
Heat interferes with multiple meiotic processes, leading to genome instability and sterility in flowering plants, including many crops. Despite its importance for food security, the mechanisms underlying heat tolerance of meiosis are poorly understood. In this study, we analyzed different meiotic processes in the Arabidopsis (Arabidopsis thaliana) accessions Columbia (Col) and Landsberg erecta (Ler), their F1 hybrids, and the F2 offspring under heat stress (37°C).
View Article and Find Full Text PDFDecoding the Nucleolar Role in Meiotic Recombination and Cell Cycle Control: Insights into Cdc14 Function.
Int J Mol Sci
November 2024
Instituto de Biología Funcional y Genómica, IBFG, CSIC-USAL, 37007 Salamanca, Spain.
The cell cycle, essential for growth, reproduction, and genetic stability, is regulated by a complex network of cyclins, Cyclin-Dependent Kinases (CDKs), phosphatases, and checkpoints that ensure accurate cell division. CDKs and phosphatases are crucial for controlling cell cycle progression, with CDKs promoting it and phosphatases counteracting their activity to maintain balance. The nucleolus, as a biomolecular condensate, plays a key regulatory role by serving as a hub for ribosome biogenesis and the sequestration and release of various cell cycle regulators.
View Article and Find Full Text PDFKeeping it safe: control of meiotic chromosome breakage.
Trends Genet
December 2024
Department of Biology, New York University, New York, NY 10003, USA. Electronic address:
Meiotic cells introduce numerous programmed DNA double-strand breaks (DSBs) into their genome to stimulate crossover recombination. DSB numbers must be high enough to ensure each homologous chromosome pair receives the obligate crossover required for accurate meiotic chromosome segregation. However, every DSB also increases the risk of aberrant or incomplete DNA repair, and thus genome instability.
View Article and Find Full Text PDFMeiotic crossovers revealed by differential visualization of homologous chromosomes using enhanced haplotype oligo-painting in cucumber.
Plant Biotechnol J
December 2024
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.
The interaction dynamics of homologous chromosomes during meiosis, such as recognition, pairing, synapsis, recombination, and segregation are vital for species fertility and genetic diversity within populations. Meiotic crossover (CO), a prominent feature of meiosis, ensures the faithful segregation of homologous chromosomes and enriches genetic diversity within a population. Nevertheless, visually distinguishing homologous chromosomes and COs remains an intractable challenge in cytological studies, particularly in non-model or plants with small genomes, limiting insights into meiotic dynamics.
View Article and Find Full Text PDFNovel BRCA1-PLK1-CIP2A axis orchestrates homologous recombination-mediated DNA repair to maintain chromosome integrity during oocyte meiosis.
Nucleic Acids Res
December 2024
Department of Integrative Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Suwon 16419, South Korea.
Double-strand breaks (DSBs) are a formidable threat to genome integrity, potentially leading to cancer and various genetic diseases. The prolonged lifespan of mammalian oocytes increases their susceptibility to DNA damage over time. While somatic cells suppress DSB repair during mitosis, oocytes exhibit a remarkable capacity to repair DSBs during meiotic maturation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!