It is effective to restrain the electroosmotic flow (EOF) with lower pH value (pH < or = 2.0). As a result, a capillary zone electrophoretic (CZE) system of lower electroosmotic flow was established. Seven aromatic amines were separated by CZE with lower electroosmotic flow. In the system, the aromatic amines combined with protons under the experimental conditions, and had positive charges. Therefore, the sample entered the capillary at the positive electrode and was detected at the negative electrode. It was found that the migration time of aromatic amines increased quickly with a slight increase in pH value in CZE at lower electroosmotic flow. The retention order of peaks of aromatic amines is related to their pKb and the numbers of amino and acidic groups. When the number of amino groups gets larger, the pKb becomes smaller, and the peaks elute earlier. The acidic groups of aromatic amines make the peaks to elute later. The optimal conditions were 40 mmol/L NaH2PO4 (pH 1.7).
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Enhancement of detoxification of xenobiotic aromatic amine dyes by N-acetyltransferase 1 (NAT1) enzyme on human keratinocytes cells through structural modification.
Environ Toxicol Pharmacol
December 2024
Department of Pharmacy and Pharmaceutical Sciences, National University of Singapore, Lower Kent Ridge Road, 4 Science Drive 2, Singapore 117544. Electronic address:
The metabolic conversion of aromatic amines to N-acetylated forms in skin and keratinocytes depends on N-acetyltransferase-1 (NAT1). Common hair color ingredient such as para-phenylenediamine (PPD) causes allergic contact dermatitis. We explored how different electronic substituents on PPD aided NAT1 enzyme biotransform oxidative arylamine (AA) compounds G1-G13 by N-acetylation, NAT-1 activity assays, metabolism, and in vitro clearance investigations in human keratinocytes, while identifying NAT-1 protein levels by Western blot and qRT-PCR.
View Article and Find Full Text PDFElucidating Ligand Interactions and Small-Molecule Activation in the Pyrrolnitrin Biosynthetic Enzyme PrnB.
J Biol Chem
December 2024
Department of Chemistry, University of Georgia, Athens, GA 30602. Electronic address:
Pyrrolnitrin, a potent antifungal compound originally discovered in Pseudomonas strains, is biosynthesized through a secondary metabolic pathway involving four key enzymes. Central to this process is PrnB, a heme enzyme that catalyzes the complex transformation of 7-Cl-L-tryptophan. Despite its structural similarity to indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) and its classification within the histidine-ligated heme-dependent aromatic oxygenase (HDAO) superfamily, PrnB has remained relatively unexplored due to challenges in reconstituting its in vitro activity.
View Article and Find Full Text PDFCan Ultrasound Replace Seeding in Flow Reactive Crystallization of an Aromatic Amine?
Org Process Res Dev
December 2024
Department of Chemical Engineering, Process Engineering for Sustainable Systems, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium.
Continuous crystallization has gained substantial interest due to its high product reproducibility, high labor efficiency, and low capital and production costs. Continuous seeding is preferable and often even required in the application of pharmaceuticals, which presents a bottleneck in continuous crystallization. This work proposes to apply ultrasound for continuous in situ seeding in the continuous reactive crystallization of an aromatic amine.
View Article and Find Full Text PDFThe sorption of algal organic matter by extracellular polymeric substances: Trade-offs in disinfection byproduct formation influenced by divalent ions.
Sci Total Environ
December 2024
College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang 550025, China. Electronic address:
Disinfection by-products (DBPs), formed from biofilm extracellular polymeric substances (EPS) and organic matter during regular disinfection practices in drinking water distribution systems, poses a potential threat to drinking water safety. However, the diverse DBP formations induced by the intertwined algal organic matter (AOM) and bacterial EPS remains elusive. In this study, we show substantial variations in EPS and DBP formation patterns driven by AOM biosorption with divalent ions (Ca and Mg).
View Article and Find Full Text PDFDeveloping dual-responsive quinolinium prodrugs of 8-hydroxyquinoline by harnessing the dual chelating sites.
Eur J Med Chem
December 2024
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology & School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China; Innovation Center for Vaccine Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address:
The bidentate metal ion chelator 8-hydroxyquinoline (8-HQ) demonstrates significant potential in anticancer therapy but is hindered by adverse effects due to nonspecific chelation in normal tissues. The phenolic hydroxyl oxygen of 8-HQ has been extensively exploited to develop O-masked 8-HQ prodrugs aimed at achieving on-demand chelation. However, the equally crucial quinoline nitrogen for chelation remains underutilized.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!