AI Article Synopsis
- RNA interference (RNAi) is a process that silences gene expression when double-stranded RNA is introduced into cells, aiding in understanding gene function.
- High throughput methods, like microarrays and microwell assays, have been developed to allow for large-scale studies in functional genomics using RNAi.
- The creation of extensive libraries of RNAi tools and various detection methods has enhanced the ability to conduct genome-wide screenings for gene function in mammalian cells.
Article Abstract
RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
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| http://dx.doi.org/10.1016/S1359-6446(04)03352-5 | DOI Listing |
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