AI Article Synopsis
- The research aimed to replace the dysfunctional Fas gene with recombinant Adenovirus containing the human Fas gene to improve keloid treatment.
- The study involved infecting fibroblasts from keloids with Adenovirus and comparing Fas protein expression levels before and after infection, revealing increased Fas expression and apoptosis.
- The findings suggest that the recombinant Adenovirus effectively enhances Fas protein expression in keloid fibroblasts and may lead to promising gene therapy approaches for keloid treatment.
Article Abstract
Objective: To replace dysfunctional Fas gene and reconstruct the blocked Fas signal by using two kinds of prepared recombinant Adenovirus which have human Fas gene .
Methods: After the keloids derived from fibroblasts were infected by the Adenovirus, the expressions of Fas protein before the exposure and after the exposure was compared. Then the function of the newly produced Fas protein was detected.
Results: The highly improve expression of Fas protein in the infected keloid derived fibroblasts was detected. Obvious apoptosis was also detected in the infected keloid derived from fibroblasts under the condition of exposing to FasMcab.
Conclusion: The recombinant Adenovirus with Fas gene can transfect the Fas gene into keloid-derived fibroblasts and highly improved the expression of Fas protein. The newly expressed Fas gene can reconstruct the blocked Fas signal. Ad-Fas (B) has better therapeutic effect in vitro gene therapy. The correlation between keloid and Fas gene was further proved and it may pave the way for further gene therapy in keloid.
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