In the present work, a label immunoquantification procedure was developed in order to determine the number of markers introduced into DNA. A non-enzymatic, in vitro labeling method for introducing the p-bromobenzoyl radical (label), through transamination and acylation reactions of the cytidine nucleotides in calf thymus DNA, was carried out. Three spacer arms with different lengths were used for separating the label from the nucleotide and three labeled DNA were obtained. Anti-p-bromobenzoyl chicken IgY polyclonal antibodies were obtained. The antibodies detected the label, into three-labeled DNA, with different sensitivities, in relation to spacer arm length used. About 3-11 labels per 4 x 10(6) bases into thermally denatured DNA were immunoquantified.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1081/PB-200041428 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biomimic models for glycemic index: Scope of sensor integration and artificial intelligence.
Food Chem X
January 2025
Division of Biochemistry, ICAR-Indian Agricultural Research Institute (IARI), New Delhi 110012, India.
The accurate quantification of glycemic index (GI) remains crucial for diabetes management, yet current methodologies are constrained by resource intensiveness and methodological limitations. digestion models face challenges in replicating the dynamic conditions of the human gastrointestinal tract, such as enzyme variability and multi-time point analysis, leading to suboptimal predictive accuracy. This review proposes an integrated technological framework combining non-enzymatic electrochemical sensing with artificial intelligence to revolutionize GI assessment.
View Article and Find Full Text PDFStudy on the In Vitro and In Vivo Antioxidant Activity and Potential Mechanism of L.
Antioxidants (Basel)
January 2025
Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou 730050, China.
Oxidative stress refers to the phenomenon in which the redox balance of the body is disrupted in response to stimuli, leading to an excessive accumulation of reactive oxygen species in vivo, which can lead to a variety of diseases. In contrast to artificial antioxidants, whose safety is controversial, natural antioxidants, which are widely available, pharmacologically active, and have little toxic side effects, are expected to be candidates for the treatment of oxidative stress-related diseases. L.
View Article and Find Full Text PDFMorphological, Physiological, and Transcriptional Changes in L. Under In Vitro Polyethylene Glycol-Induced Water Stress.
Biology (Basel)
January 2025
Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur 176061, Himachal Pradesh, India.
Saffron ( L.), a perennial geophyte from the Iridaceae family, blooms in autumn and thrives in Mediterranean-like climates. It is highly valued for its therapeutic and commercial uses.
View Article and Find Full Text PDFAmeliorative effect of aqueous leaf extract of Pistacia lentiscus L. against oxaliplatin-induced hepatic injury, oxidative stress, and DNA damage in vitro and in vivo.
Med Oncol
January 2025
Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Jijel, 18000, Jijel, Algeria.
The current study aimed to assess the preventive effects of aqueous leaf extract of Pistacia lentiscus (ALEPL) against Oxaliplatin (OXA)-induced DNA damage, hepatic injury, and oxidative stress. The in vitro cytotoxic and genotoxic effects of OXA and ALEPL on HCT116 colon cancer cells were evaluated using the MTT (Tetrazolium salt reduction) assay and comet assay. The in vivo study involved 24 female NMRI (Naval Medical Research Institute) mice that were equally divided into four groups as follows: Control group, ALEPL-treated group (100 mg/kg), OXA-treated group (7 mg/kg), and ALEPL-treated group (100mg/kg) + OXA (7mg/kg).
View Article and Find Full Text PDFAGEing of collagen: The effects of glycation on collagen's stability, mechanics and assembly.
Matrix Biol
February 2025
Department of Physics, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada. Electronic address:
Advanced Glycation End Products (AGEs) are the end result of the irreversible, non-enzymatic glycation of proteins by reducing sugars. These chemical modifications accumulate with age and have been associated with various age-related and diabetic complications. AGEs predominantly accumulate on proteins with slow turnover rates, of which collagen is a prime example.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!