AI Article Synopsis
- The study focused on three cell lines from Saussurea medusa to assess their ability to produce jaceosidin and hispidulin, with the red cell line showing the highest yields of 75 mg/l and 6.4 mg/l, respectively.
- Production levels were directly linked to the size of compact callus aggregates (CCAs) and the overall health of the cells.
- The red cell line exhibited optimal CCAs of 2-4 mm in diameter, which constituted 64% of the biomass, and maintained good cell viability over 10 sub-cultures.
Article Abstract
Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2-4 mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10529-004-6934-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ethyl acetate extract of Artemisia argyi improves the resistance of cotton to Verticillium dahliae by activating the immune response.
Plant Physiol Biochem
December 2024
School of Pharmacy, Hubei University of Chinese Medicine, 430065, Wuhan, China; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, 430065, Wuhan, China. Electronic address:
Verticillium wilt, a significant pathogen affecting cotton, has historically been challenging to control, posing a substantial threat to the sustainable development of the cotton industry. This study demonstrates that resistance to Verticillium dahliae in cotton can be enhanced by treating the roots with an ethyl acetate extract (EAAA) extracted from Artemisia argyi. The mechanisms by which EAAA activates immunity in cotton were elucidated by examining the expression levels of resistance genes post-treatment, evaluating salicylic acid (SA) and jasmonic acid (JA) levels, analyzing transcriptome data, and employing virus-induced gene silencing (VIGS) technology.
View Article and Find Full Text PDFScreening Anti-Rheumatoid Arthritis Synovitis Effective Ingredients of Total Flavonoid From Artemisia argyi Folium Based on Spectrum-Effect Relationship.
Phytochem Anal
November 2024
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Introduction: Flavonoids are the main nonvolatile component responsible for the anti-rheumatoid arthritis (RA) synovitis activities of Artemisia argyi Folium. However, the effective ingredient remains unidentified. Spectrum-effect relationships analysis was a reliable and efficient strategy for herbal effective ingredient discovery.
View Article and Find Full Text PDFSecondary Metabolites and Their Biological Evaluation from the Aerial Parts of Sibth. & Sm. (Asteraceae).
Int J Mol Sci
October 2024
Laboratory of Pharmacognosy, Faculty of Health Sciences, School of Pharmacy, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Phytochemical investigation of Sibth. & Sm. resulted in the isolation of twenty-two natural products: eleven sesquiterpene lactones, artemorin (), tamirin (), tanachin (), reynosin (), baynol C (), desacetyl-β-cyclopyrethrosin (), 1β-hydroxy-4α-methoxy-5α,7α,6β-eudesm-11(13)-en-6,12-olide (), 1β,4α,6α-trihydroxyeudesm-11-en-8α,12-olide (), 1β-hydroxy-arbusculin A (), methyl-1β,4α,6α-trihydroxy-5α,7αH-eudesm-11(13)-en-12-oate () and methyl-1β,6α,8α-trihydroxy-5α,7αH-eudesma-4(15),11(13)-dien-12-oate (); one lignan, pinoresinol (); one norisoprenoid, loliolide (); six flavonoids (four genins and two glycosides), hispidulin (), nepetin (), jaceosidin (), eriodictyol (), eriodictyol-3'-O-β-D-glucoside () and eriodictyol-7-O-β-D-glucuronide (); and three phenolic derivatives (one phenolic acid and two phenolic glucosides), protocatechuic acid (), arbutin () and nebrodenside A ().
View Article and Find Full Text PDFDual SARS-CoV-2 and MERS-CoV inhibitors from : isolation, structure elucidation, molecular modelling studies, and activities.
Org Biomol Chem
August 2024
Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
The COVID-19 pandemic has spread throughout the whole globe, so it is imperative that all available resources be used to treat this scourge. In reality, the development of new pharmaceuticals has mostly benefited from natural products. The widespread medicinal usage of species in the Asteraceae family is extensively researched.
View Article and Find Full Text PDF[Corythucha marmorata affects growth and quality of Artemisia argyi].
Zhongguo Zhong Yao Za Zhi
October 2023
Pharmacy Faculty, Hubei University of Chinese Medicine Wuhan 430065, China.
This study aims to investigate the impact of the invasive pest Corythucha marmorata on the growth and quality of Artemi-sia argyi. The signs of insect damage at the cultivation base of A. argyi in Huanggang, Hubei were observed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!