Mechanical strain initiates a variety of responses in pulmonary epithelial cells. The signaling pathways and molecular alterations leading to these responses remain unclear To identify novel signal transduction pathways activated by strain, macroarray analysis was performed on strained pulmonary epithelial cells. Glutathione S-transferase (GST) pi, GST mu, and heat shock protein (HSP)-27 were increased by strain. Western blotting verified that increases in cDNA of these redox-related molecules resulted in an increase in protein. Phosphorylation of HSP-27 was increased after strain, further supporting the role of HSP-27 in strain-induced signal transduction. Strain-induced oxidative stress was verified with the oxidant-sensitive dye dichlorodihydrofluorescein diacetate.
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