AI Article Synopsis
- Prominin-1 is a cell-surface glycoprotein originally discovered in mouse neuroepithelial stem cells, featuring five transmembrane domains and localizing to membrane protrusions.
- Human Prominin-1, identified through a specific monoclonal antibody (AC133), is found on hematopoietic stem cells and is produced from multiple alternatively spliced mRNA isoforms driven by different tissue-specific promoters.
- While the exact biological function of Prominin-1 remains unclear, the AC133 epitope is valuable for isolating hematopoietic and endothelial progenitor cells in research.
Article Abstract
Prominin-1, originally found on neuroepithelial stem cells in mice, is a five transmembrane domain cell-surface glycoprotein that localizes to membrane protrusions. Its homologue human Prominin-1 was first isolated from hematopoietic stem cells by a monoclonal antibody recognizing a specific epitope designated as AC133 (CD133). Transcription of Prominin-1 is driven by five tissue-specific alternative promoters resulting in the formation of differentially spliced mRNA isoforms. Prominin-1 is expressed on different types of stem cells, but it is not known if it plays a significant role in key stem cell functional features. Although the biological function of Prominin-1 is not well understood, the AC133 epitope currently serves as a useful marker for the isolation of hematopoietic and endothelial progenitor cells.
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| http://dx.doi.org/10.1016/j.biocel.2004.08.010 | DOI Listing |
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