AI Article Synopsis
- Shewanella oneidensis MR-1 can thrive in a variety of temperatures, with optimal growth at around 35°C, but can also grow in near-freezing conditions.
- At room temperature (22°C), MR-1 has a doubling time of about 40 minutes, but this significantly increases to about 67 hours when the temperature drops to 3°C, leading to a long lag phase of over 100 hours.
- Cold-grown MR-1 cells exhibit unique characteristics such as long motile filaments, different protein production, changes in cellular lipid patterns, and the appearance of pilus-like structures when transitioning back to warmer temperatures.
Article Abstract
Shewanella oneidensis MR-1 is a mesophilic bacterium with a maximum growth temperature of approximately 35 degrees C but the ability to grow over a wide range of temperatures, including temperatures near zero. At room temperature ( approximately 22 degrees C) MR-1 grows with a doubling time of about 40 min, but when moved from 22 degrees C to 3 degrees C, MR-1 cells display a very long lag phase of more than 100 h followed by very slow growth, with a doubling time of approximately 67 h. In comparison to cells grown at 22 degrees C, the cold-grown cells formed long, motile filaments, showed many spheroplast-like structures, produced an array of proteins not seen at higher temperature, and synthesized a different pattern of cellular lipids. Frequent pilus-like structures were observed during the transition from 3 to 22 degrees C.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC546687 | PMC |
| http://dx.doi.org/10.1128/AEM.71.2.811-816.2005 | DOI Listing |
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