AI Article Synopsis
- The study compares two methods for detecting Helicobacter species in mouse intestine samples: genus-specific PCR-DGGE and species-specific PCR.
- Findings indicate that genus-specific PCR-DGGE might not identify all Helicobacter species present in samples with multiple species.
- The effectiveness of genus-specific PCR-DGGE is influenced by the initial ratio of template DNA in the samples.
Article Abstract
The detection of Helicobacter species by genus-specific polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was compared with that by species-specific PCR in murine intestinal samples. Results suggest that, in samples containing multiple Helicobacter species, genus-specific PCR-DGGE may fail to detect all Helicobacter species present and that this relates to the initial template DNA ratio.
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| http://dx.doi.org/10.1111/j.1523-5378.2005.00294.x | DOI Listing |
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