Objectives: Hypertonic saline is recommended for the induction of sputum to assess airway inflammation. Methacholine is most commonly used to assess airway responsiveness. The aim of this study was to compare sputum induction by methacholine and hypertonic saline challenges, and the subsequent inflammatory cell differential counts.
Methodology: Subjects (n = 56) underwent inhaled hypertonic saline (4.5% for < or = 15 min without bronchodilator pretreatment) and methacholine (maximum cumulative dose 4 micromol) challenges 1 week apart, in random order. Sputum was processed, and on cytospins stained with May-Grunwald-Giemsa, a total of 400 inflammatory plus squamous cells were counted to obtain a differential cell count.
Results: A satisfactory sputum sample was obtained from 29 subjects following hypertonic saline and also from 10 subjects following methacholine. For these 10 subjects, the mean percent macrophages, neutrophils, eosinophils, and lymphocytes were similar after methacholine (64%, 26%, 7% and 1%) or hypertonic saline (67%, 26%, 6% and 1%). The results were similar for squamous cells (42% and 45%) and when asthmatics and nonasthmatics were compared separately.
Conclusions: Inhaled methacholine has a much lower yield of sputum induction compared with hypertonic saline. However, the differential sputum cell count is similar following both challenge agents.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1440-1843.2005.00632.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Necrotizing fasciitis of the breast in a breastfeeding mother: A case report.
Int J Surg Case Rep
January 2025
Department of Surgery, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka. Electronic address:
Introduction: Necrotizing fasciitis (NF) is a rapidly progressive life-threatening infection involving necrosis of subcutaneous tissues and fascia. Though it mainly affects the extremities, perineum and abdominal wall, it rarely affects the breast.
Presentation Of Case: A 30-year-old woman on postpartum day seven presented with one-day history of fever with chills and rigors and painful, swollen and tender left breast.
Hydatid cysts, caused by the parasite, predominantly affect the liver and lungs, but can also impact other organs such as the kidneys, brain, and muscles. Infection occurs when individuals ingest eggs from contaminated food or water, leading to cyst formation primarily in the liver. While hydatid cysts are commonly found in various endemic regions, muscular involvement is rare, particularly in the psoas muscle.
View Article and Find Full Text PDFInduced Sputum: A Valuable Tool for Assessing Cellular and Microbiological Characteristics in Cystic Fibrosis Expectorating Teenagers.
Pediatr Pulmonol
January 2025
Centre de Ressources et de Compétences pour la Mucoviscidose, CHU Timone-Enfants, Marseille, France.
Introduction: Cellular characteristics of induced sputum (IS) are not investigated in cystic fibrosis (CF) patients.
Objectives: This pilot study, conducted on 17 expectorating CF adolescents, compared sputa obtained the same day, in a stable period, by autogenic drainage (expectorating sputum, ES) and 4 h later after inhaling hypertonic saline (IS).
Results: No difference was noted concerning weight, volume, and percentage of dead cells between the two collection methods.
A multimedia tool for infection prevention and control practices in the intensive care unit: a participatory interventional before-after study.
Infect Prev Pract
March 2025
Cardio Thoracic and Vascular Surgery, PGIMER, Chandigarh, India.
Background: Infection prevention and control (IPC) practices by critical care nurses are crucial in preventing ventilator-associated pneumonia (VAP) and central-line-associated bloodstream infection (CLABSI).
Aim: To implement an integrative approach to developing a set of IPC practices and disseminating information on the IPC practices through an educational multimedia tool to improve compliance with the practices.
Methods: This participatory interventional before-after study was conducted in a single tertiary care centre's cardiac surgical intensive care unit (ICU) from May 2022 to March 2023.
Efficacy and Renal Safety of Protocol-based 11.7% Hypertonic Saline Infusion Compared with 20% Mannitol in Patients with Elevated Intracranial Pressure: A Study Protocol for a Randomized Clinical Trial.
Electrolyte Blood Press
December 2024
Department of Internal Medicine, College of Medicine, Dankook University, Republic of Korea.
Background: Elevated intracranial pressure (ICP) is a potentially life-threatening condition requiring prompt intervention. While both mannitol and hypertonic saline (HTS) are commonly used hyperosmotic agents for treating elevated ICP, there is insufficient evidence comparing their renal safety profiles and overall effectiveness. This study protocol outlines a pragmatic randomized trial to compare protocol-based 11.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!