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Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA.
Diagnostics (Basel)
November 2012
DTU-FOOD, Laboratory of Applied Micro-Nanotechnology (LAMINATE), Diagnostic Engineering, Division of Food Microbiology, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8.
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