The X-ray structure of a partly self-complementary peptide nucleic acid (PNA) decamer (H-GTAGATCACT-l-Lys-NH(2)) to 2.60 A resolution is reported. The structure is mainly controlled by the canonical Watson-Crick base pairs formed by the self-complementary stretch of four bases in the middle of the decamer (G(4)A(5)T(6)C(7)). One right- and one left-handed Watson-Crick duplex are formed. The two PNA units C(9)T(10) change helical handedness, so that each PNA strand contains both a right- and a left-handed section. The changed handedness in C(9)T(10) allows formation of Hoogsteen hydrogen bonding between C(9)T(10) and G(4)A(5) of a PNA strand in an adjacent Watson-Crick double helix of the same handedness. Thereby, a PNA-PNA-PNA triplex is formed. The PNA unit A(3) forms a noncanonical base pair with A(8) in a symmetry-related strand of opposite handedness; the base pair is of the A-A reverse Hoogsteen type. The structural diversity of this PNA demonstrates how the PNA backbone is able to adapt to structures governed by the stacking and hydrogen-bonding interactions between the nucleobases. The crystal structure further shows how PNA oligomers containing limited sequence complementarity may form complex hydrogen-bonding networks.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja0458726 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Pooled Pharmacokinetic Analysis for Piperacillin/Tazobactam Across Different Patient Populations: From Premature Infants to the Elderly.
Clin Pharmacokinet
December 2024
Department of Anesthesiology, University of Groningen, University Medical Center Groningen, P. O. Box 30001, 9700 RB, Groningen, The Netherlands.
Background And Objectives: The pharmacokinetics (PK) of piperacillin/tazobactam (PIP/TAZ) is highly variable across different patient populations and there are controversies regarding non-linear elimination as well as the fraction unbound of PIP (f). This has led to a plethora of subgroup-specific models, increasing the risk of misusing published models when optimising dosing regimens. In this study, we aimed to develop a single model to simultaneously describe the PK of PIP/TAZ in diverse patient populations and evaluate the current dosing recommendations by predicting the PK/pharmacodynamics (PD) target attainment throughout life.
View Article and Find Full Text PDFRecognition of Noncanonical RNA Base Pairs Using Triplex-Forming Peptide Nucleic Acids.
ACS Chem Biol
December 2024
Department of Chemistry, Binghamton University, The State University of New York, Binghamton, New York 13902, United States.
Noncanonical base pairs play an important role in enabling the structural and functional complexity of RNA. Molecular recognition of such motifs is challenging because of their diversity, significant deviation from the Watson-Crick structures, and dynamic behavior, resulting in alternative conformations of similar stability. Triplex-forming peptide nucleic acids (PNAs) have emerged as excellent ligands for the recognition of Watson-Crick base-paired double helical RNA.
View Article and Find Full Text PDFAchieving mainstream nitrogen removal by partial nitrification and anammox in the carriers-coupled membrane aerated biofilm reactor.
Water Res
December 2024
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China; Research and Application Centre for Membrane Technology, School of Environment, Tsinghua University, Beijing, 100084, China. Electronic address:
The integration of partial nitrification-anammox (PN/A) into membrane-aerated biofilm reactor (MABR) is a promisingly energy-efficient and high-efficiency technology for nitrogen removal. The inhibition of nitrite oxidizing bacteria (NOB) remains as the most significant challenge for its development. In our investigation, we proposed a novel process to integrate carriers to MABR (CMABR), which combined the carriers enriched with anaerobic ammonium-oxidizing bacteria (AnAOB) and partial nitrifying MABR system.
View Article and Find Full Text PDFA Retrospective Analysis of Mortality Due to Pneumonia and Renal Disease in a Midwestern Patient Population.
Cureus
November 2024
Primary Care, Kansas City University, Joplin, USA.
Background: The impact of pneumonia (PNA) with concomitant renal disease (RD) has not been fully investigated in a United States Midwestern patient population, despite the morbidity and mortality associated with such diseases.
Materials And Methods: A retrospective cohort study was performed on International Classification of Diseases, 10 Revision (ICD-10) data from a hospital system located in Southwest Missouri. Data was acquired from patients admitted between January 2019 and December 2021.
State-dependent inter-network functional connectivity development in neonatal brain from the developing human connectome project.
Dev Cogn Neurosci
December 2024
Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, China. Electronic address:
Although recent studies have consistently reported the emergence of resting-state networks in early infancy, the changes in inter-network functional connectivity with age are controversial and the alterations in its dynamics remain unclear at this stage. This study aimed to investigate dynamic functional network connectivity (dFNC) using resting-state functional MRI in 244 full-term (age: 37-44 weeks) and 36 preterm infants (age: 37-43 weeks) from the dHCP dataset. We evaluated whether early dFNC exhibits age-dependent changes and is influenced by preterm birth.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!