Glial cell line-derived neurotrophic factor (GDNF) is a transforming growth factor-beta which has shown beneficial effects in rats after acute focal cerebral ischemia (FCI). To study the effects of GDNF on chronic FCI injury in conscious rats, we used fibrin glue (GDNF-fibrin glue) and fibrin glue free (GDNF-only)-GDNF topically applied to the ischemic brain after right middle cerebral artery (MCA) ligation. Infarct brain volume and functional motor deficits were measured before and after FCI injury. After FCI injury induced by right MCA ligation, rats were randomly assigned to one of four treatment groups: (a) sham, (b) control, (c) topically applied GDNF (1 mug)-only, and (d) topically applied GDNF (1 mug)-fibrin glue. The degree of ischemic brain injury was estimated by infarct volume of right MCA territory at 4 weeks after occlusion. The functional motor deficits were quantified with rotarod test and grasping power test once a week. Topically applied GDNF-fibrin glue at infarct brain tissue after 4 weeks FCI injury significantly reduced the total infarct volume by 44.3% and 36%, respectively, compared to that of control group and GDNF-only group. The mean latencies for rats to stay on the rotarod were 55.0%, 50.3%, and 92.2% (P < 0.05 vs. control group and GDNF-only group) of baseline, respectively, in the control, GDNF-only, and GDNF-fibrin glue groups at the end of the 1st week after FCI injury but 75.3%, 67.3%, and 106.6% (P < 0.05 vs. control group and GDNF-only group) of baseline at the end of the 4th week after FCI injury. The mean values of grasping power were 78.7%, 71.7%, and 101.2% (P < 0.05 vs. control group and GDNF-only group) of baseline, respectively, in the control, GDNF-only, and GDNF-fibrin glue groups at the end of 1st week after FCI injury but 89.6%, 97.6%, and 120.7% (P < 0.05 vs. control group) of baseline at the end of 4th week after FCI injury. These results indicate that GDNF-fibrin glue not only reduced the total infarct volume after FCI injury but can also improve motor deficits after FCI injury. We concluded GDNF-fibrin glue could facilitate delivery of GDNF to the damaged brain tissue with subsequent reduction of ischemic brain injury accompanied by enhancing functional recovery in rats with chronic FCI injury.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.brainres.2004.10.067 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Effects of electroacupuncture on expression of miR-218 and HMGB1/TLR4 signaling pathway in rats with focal cerebral ischemia].
Zhen Ci Yan Jiu
November 2024
College of Acupuncture-Moxibustion and Tuina, Anhui University of Chinese Medicine, Hefei 230012.
Objectives: To observe the effect of electroacupuncture (EA) on high mobility group box 1 (HMGB1) / toll-like receptor 4 (TLR4) signaling pathway and inflammatory injury in rats with focal cerebral ischemia (FCI), so as to explore its mechanisms underlying amelioration of ischemic stroke.
Methods: Male SD rats were randomly divided into sham operation (=18), model (=18) and EA (=18) groups. The FCI model was established according to Longa's method.
Mixed-variable graphical modeling framework towards risk prediction of hospital-acquired pressure injury in spinal cord injury individuals.
Sci Rep
October 2024
Department of Health Science and Technology, ETH Zürich, 8006, Zürich, Switzerland.
Developing machine learning (ML) methods for healthcare predictive modeling requires absolute explainability and transparency to build trust and accountability. Graphical models (GM) are key tools for this but face challenges like small sample sizes, mixed variables, and latent confounders. This paper presents a novel learning framework addressing these challenges by integrating latent variables using fast causal inference (FCI), accommodating mixed variables with predictive permutation conditional independence tests (PPCIT), and employing a systematic graphical embedding approach leveraging expert knowledge.
View Article and Find Full Text PDFInfluenza A virus replicates productively in primary human kidney cells and induces factors and mechanisms related to regulated cell death and renal pathology observed in virus-infected patients.
Front Cell Infect Microbiol
April 2024
Institute of Medical Virology, Justus Liebig University Giessen, Giessen, Germany.
Article Synopsis
- The study investigates how the Influenza A virus (IAV) affects human kidney cells, specifically looking at the mechanisms behind acute kidney injury (AKI) during IAV infection.
- Researchers conducted various experiments, including infectivity assays and transcriptome/proteome analyses, revealing that IAV can replicate effectively in kidney cells.
- Findings suggest that regulated cell death plays a key role in kidney damage due to IAV, highlighting potential targets for treatment to mitigate kidney-related complications.
Lumbosacral transitional vertebra in 14 dog breeds in Norway: Occurrence, risk factors and association with hip dysplasia.
Vet J
February 2024
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway; Veterinærradiologene AS, Skytta terrasse 2, 1481 Hagan, Norway.
A lumbosacral transitional vertebra (LTV) is a congenital anomaly of the spine and has been suggested to predispose to canine hip dysplasia (CHD). This retrospective, cross-sectional study investigated the prevalence of LTV and CHD among 14 dog breeds in Norway, the possible associations with risk factors, and whether LTV was a risk factor for the development of hip dysplasia. The results were based on evaluation of ventrodorsal radiographs from the CHD screening program from the Norwegian Kennel Club from February 2014 to January 2022.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!