AI Article Synopsis
- The PP2 gene promoter from pumpkins was isolated and studied for its activity and specificity in phloem tissue using transgenic tobacco plants with a GUS reporter.
- A key 473-bp fragment of the promoter, known as NPII, demonstrated strong activity and maintained phloem specificity after deletion analysis.
- An enhanced version of the NP promoter was developed by duplicating a regulatory sequence, showing superior activity in phloem compared to other well-known viral promoters.
Article Abstract
The promoter of the pumpkin (Cucurbita moschata) PP2 gene (designated NP) was isolated from the restriction enzyme-digested genomic DNA pool by genome walking and its activity and phloem specificity were examined in transgenic tobacco plants by using GUS as a reporter. Deletion analysis of the promoter revealed that the 473-bp fragment (-465 to + 8 relative to the transcription start site; designated as NPII) exhibited similar activity as the full-length NP promoter and retained its phloem specificity. Furthermore, the sequence from -465 to -171 was shown to contain positive regulatory cis-elements for the promoter activity. An enhanced NP promoter was constructed by duplicating the sequence -465 to -85, and its activity in phloem tissue was shown to be higher than that of the Commelina Yellow Mottle Virus (CoYMV) promoter or a chimeric promoter consisting of the double enhancer sequence from the Cauliflower Mosaic Virus (CaMV) 35S promoter fused upstream to the NPII fragment.
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| http://dx.doi.org/10.1007/s11248-004-2738-2 | DOI Listing |
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