AI Article Synopsis
- The study aimed to explore how interleukin-10 (IL-10) affects the activation of hepatic stellate cells (HSC) via specific cellular pathways.
- HSC were divided into four groups, with varying concentrations of IL-10 administered to three of the groups, and analyses were performed to measure the expression of certain proteins involved in cell signaling.
- Results showed that increased IL-10 levels significantly reduced the expression of proteins linked to cell activation in a dose-dependent manner, indicating that IL-10 inhibits HSC activation through the PDGF/MAPK pathway.
Article Abstract
Objective: To investigate the regulatory effect of interleukin-10 (IL10) on the activation of hepatic stellate cells (HSC) through platelet derived growth factor (PDGF) and mitogen-activated protein kinase (MAPK) pathways.
Methods: HSC were divided randomly into 4 groups. Group 1 served as a control. HSC were incubated with 1 ng/ml, 5 ng/ml, and 25 ng/ml IL-10 in groups 2, 3 and 4. RT-PCR and western blot were used to detect the expression of PDGF and MAPK protein ERK and p38 and alpha-SMA.
Results: Compared with the control group, expressions of ERK, p38 and alpha-SMA of groups 2, 3 and 4 were significantly lower (F values were 240.47, 21.39, 28.86 respectively. IL-10 inhibited PDGF and MAPK protein ERK and p38 and alpha-SMA expression in a dose-dependent way.
Conclusion: IL-10 inhibits activation of HSC through the PDGF/MAPK pathway.
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