Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method.

FEMS Microbiol Lett

Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.

Published: February 2005

Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.femsle.2004.12.014DOI Listing

Publication Analysis

Top Keywords

lamp method
12
shigella enteroinvasive
8
enteroinvasive escherichia
8
escherichia coli
8
loop-mediated isothermal
8
isothermal amplification
8
sensitive rapid
4
rapid detection
4
detection shigella
4
coli loop-mediated
4

Similar Publications

Background And Aims: Rhinoplasty, a prevalent cosmetic surgery, often involves lateral osteotomy, which can lead to ocular complications such as edema and ecchymosis. A potential complication is increased intraocular pressure (IOP) postoperatively. This study aims to investigate the impact of lateral osteotomy during rhinoplasty on IOP in patients.

View Article and Find Full Text PDF

Advances and Challenges in the Diagnosis of Leishmaniasis.

Mol Diagn Ther

January 2025

Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.

Leishmaniasis remains a significant public health challenge, particularly in endemic regions with limited resources. Traditional diagnostic methods, including microscopy, culture, and serology, though widely utilized, often suffer from limitations such as variable  sensitivity, time delays, and the need for specialized infrastructure. Some of these limitations have been addressed with the emergence of molecular diagnostic techniques.

View Article and Find Full Text PDF

Asperulosidic acid inhibits the PI3K/Akt/NF-κB pathway to suppress endotoxin-induced uveitis.

Front Med (Lausanne)

January 2025

Department of Ophthalmology, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Introduction: Uveitis, a severe inflammatory disease affecting the uvea, is associated with visual impairment and irreversible blindness. Asperulosidic Acid (ASPA), derived from , is known for its notable anti-inflammatory and antioxidant characteristics.

Methods: The present study explored the potential anti-inflammatory effects and the fundamental processes of ASPA by injecting it or a placebo into the vitreous of rats with endotoxin-induced uveitis (EIU).

View Article and Find Full Text PDF

Background: Anterior uveitis is a common manifestation in individuals with rheumatic conditions such as spondylarthritis, Behçet's syndrome, juvenile idiopathic arthritis, and sarcoidosis. Clinical differentiation between granulomatous and non-granulomatous corneal endothelial exudates is crucial to subsequent diagnosis and treatment. Anterior segment optical coherence tomography (AS-OCT) can ensure an accurate differential diagnosis and appropriate follow-up after local and systemic therapy.

View Article and Find Full Text PDF

A novel ready-to-use loop-mediated isothermal amplification (LAMP) method for detection of Burkholderia mallei and B. pseudomallei.

BMC Microbiol

January 2025

Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo, Hokkaido, 060-0818, Japan.

Background: Glanders and melioidosis are contagious zoonotic diseases caused by Burkholderia mallei and B. pseudomallei, respectively. Bacterial isolation and polymerase chain reaction (PCR) have been used to detect these bacteria in animals suspected of infection; however, both methods require skilled experimental techniques and expensive equipment.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!