A mutant of Desulfovibrio vulgaris Hildenborough lacking a gene for [NiFe] hydrogenase was generated. Growth studies, performed for the mutant in comparison with the wild-type, showed no strong differences during the exponential growth phase. However, the mutant cells died more rapidly in the stationary growth phase.
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http://dx.doi.org/10.1042/BST0330059 | DOI Listing |
J Bacteriol
December 2024
School of Biological Sciences, University of Oklahoma, Norman, Oklahoma, USA.
Unlabelled: Ubiquitous in nature, biofilms provide stability in a fluctuating environment and provide protection from stressors. Biofilms formed in industrial processes are exceedingly problematic and costly. While biofilms of sulfate-reducing bacteria in the environment are often beneficial because of their capacity to remove toxic metals from water, in industrial pipelines, these biofilms cause a major economic impact due to their involvement in metal and concrete corrosion.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Understanding the extracellular electron transfer mechanisms of electroactive bacteria could help determine their potential in microbial fuel cells (MFCs) and their microbial syntrophy with redox-active minerals in natural environments. However, the mechanisms of extracellular electron transfer to electrodes by sulfate-reducing bacteria (SRB) remain underexplored. Here, we utilized double-chamber MFCs with carbon cloth electrodes to investigate the extracellular electron transfer mechanisms of Hildenborough (H), a model SRB, under varying lactate and sulfate concentrations using different H mutants.
View Article and Find Full Text PDFSulfate-reducing microorganisms extensively contribute to the corrosion of ferrous metal infrastructure. There is substantial debate over their corrosion mechanisms. We investigated Fe corrosion with , the sulfate reducer most often employed in corrosion studies.
View Article and Find Full Text PDFJ Chem Inf Model
May 2024
Institute of Chemistry, Technische Universität Berlin, Straße des 17. Juni 135, 10623 Berlin, Germany.
[NiFe] hydrogenases can act as efficient catalysts for hydrogen oxidation and biofuel production. However, some [NiFe] hydrogenases are inhibited by gas molecules present in the environment, such as O and CO. One strategy to engineer [NiFe] hydrogenases and achieve O- and CO-tolerant enzymes is by introducing point mutations to block the access of inhibitors to the catalytic site.
View Article and Find Full Text PDFBiochemistry
September 2023
Department of Chemistry and Biochemistry, The Ohio State University, 100 W 18th Avenue, Columbus, Ohio 43210, United States.
Nickel-substituted rubredoxin (NiRd) from has previously been shown to act as both a structural and functional mimic of the [NiFe] hydrogenase. However, improvements both in turnover frequency and overpotential are needed to rival the native [NiFe] hydrogenase enzymes. Characterization of a library of NiRd mutants with variations in the secondary coordination sphere suggested that protein dynamics played a substantial role in modulating activity.
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