We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40 degrees C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.
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It is shown, that the producer of exopolysaccharides Xantomonas campestris pv. campestris IMV B-7001 is more sensitive to freeze-drying, than to freezing-thawing and keeps stable morphological properties. Predicted preservation of viability is within the limits of 2-8 years at -70 degrees C and exceeds 30 years at -135 degrees C and freeze-drying.
View Article and Find Full Text PDFLymphocyte resistance to cold anabiosis of different degree.
Bull Exp Biol Med
August 2006
Laboratory of Blood Cryophysiology, Institute of Physiology, Komi Research Center, Ural Division of Russian Academy of Sciences, Syktyvkar.
We studied the resistance of human blood T and B lymphocytes to cold anabiosis (-20 degrees C, -40 degrees C, and -80 degrees C) of different duration.
View Article and Find Full Text PDF[Introduction of human leukocytes in cold anabiosis (-20 degrees C) under an exponential program].
Ross Fiziol Zh Im I M Sechenova
May 2005
Investigation of effective and accessible method for administration of human blood leukocytes in the cold anabiosis condition at am oderately low temperature of minus 20 degrees C by the exponential program with cryopreservative which does not require washing from the bio-object after its thawing, has been proposed. Morphological and functional properties of the leukocytes after different periods of conservation in the state of cold anabiosis were studied. The data obtained suggest that optimal conservation of the morpho-functional properties of cells with the aid of this method equals 3 weeks.
View Article and Find Full Text PDFPhagocytic activity of neutrophils and lymphocytes at various periods of storage in the state of cold anabiosis (-40 degrees C).
Bull Exp Biol Med
October 2004
Laboratory for Cryophysiology of Blood, Institute of Physiology, Komi Research Center, Ural Division of the Russian Academy of Sciences, Syktyvkar.
We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40 degrees C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.
View Article and Find Full Text PDFA method for introducing human blood leukocytes in cold anabiosis at -50 degrees Celsius.
Bull Exp Biol Med
November 2003
Laboratory of Blood Kryophysiology, Institute of Physiology, Komi Research Center, Ural Division of Russian Academy of Medical Sciences, Syktyvkar, Russia.
We propose a simple, effective, and economic method for introducing human blood leukocytes in cold anabiosis at moderately low temperature (-50 degrees Celsius).
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