Optimum culture conditions for the production of N-substituted formamide deformylase by Arthrobacter pascens F164.

Biosci Biotechnol Biochem

Institute of Applied Biochemistry, and Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.

Published: January 2005

AI Article Synopsis

  • The study focused on finding the best growing conditions for a new enzyme called N-substituted formamide deformylase in the bacteria Arthrobacter pascens F164.
  • The highest enzyme activity was achieved by using a synthetic medium where N-benzylformamide was the only nitrogen source.
  • The enzyme is inducible, meaning its production is stimulated specifically by the presence of N-benzylformamide.

Article Abstract

We investigated the optimum culture conditions for the production of a novel enzyme, N-substituted formamide deformylase, which acts mainly on N-benzylformamide, in Arthrobacter pascens F164. The highest enzyme activity was obtained when this strain F164 was cultivated in a synthetic medium with N-benzylformamide as sole nitrogen source. This deformylase was found to be an inducible enzyme depending on N-benzylformamide.

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http://dx.doi.org/10.1271/bbb.69.228DOI Listing

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