Chikungunya virus (CHIKV), a member of the alphavirus genus, is of considerable public health concern in Southeast Asian and African countries. However, despite serological evidence, the diagnosis of this arthropod-borne human disease is confirmed infrequently and needs to be improved. In fact, illness caused by CHIKV can be confused with diseases such as dengue or yellow fever, based on the similarity of the symptoms, and laboratory confirmation of suspected cases is required to launch control measures during an epidemic. Moreover, no quantitative molecular tool is described to study CHIKV replication or detection in clinical samples and cell culture supernatants. In this study, a specific and sensitive CHIKV one-step TaqMan RT-PCR assay was developed as a tool for the diagnosis of African CHIKV as well as a rapid indicator of active infection by quantifying viral load. This study also showed that a simple heat viral RNA release during the reverse transcription step constituted an alternative to the conventional RNA extraction method.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jviromet.2004.11.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Simple and Sensitive RT-qPCR Technology for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus.
Vet Sci
January 2025
College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China.
To establish a rapid and sensitive detection method for the porcine reproductive and respiratory syndrome virus (PRRSV), gene-specific primers and a TaqMan probe were designed based on the gene of PRRSV, and a new stable fully pre-mixed reverse transcription real-time fluorescence quantitative PCR (RT-qPCR) reaction mixture was developed. A simple and rapid RT-qPCR detection method for PRRSV was developed by optimizing nucleic acid amplification conditions. The results showed that the method was able to specifically detect PRRSV without cross-reactivity with the other 11 porcine susceptible viruses.
View Article and Find Full Text PDFPhylogenetic and Sequence Analyses of the Variable Region in the Glycoprotein Gene of the Respiratory Syncytial Virus Isolated from Iraqi Patients.
Malays J Med Sci
December 2024
Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
Background: Respiratory syncytial virus (RSV) is a common aetiological agent that causes respiratory infections, especially among infants. Identifying circulating RSV genotypes is an essential strategy for understanding the spread of the virus in a certain area. Sequencing the variable regions of the attachment glycoprotein (G) gene of RSV is a quick and direct approach for identifying the genotypes.
View Article and Find Full Text PDFDevelopment and Clinical Detection of Rapid Molecular Diagnostic System for Pathogenic Dermatophytes of Tinea Capitis of Multiple Centres in China.
Mycoses
January 2025
Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China.
Objectives: Tinea capitis remains a common fungal infection in children worldwide. Species identification is critical for determining the source of infection and reducing transmission. In conventional methods, macro- and microscopic analysis is time-consuming and results in slow fungal growth or low specificity.
View Article and Find Full Text PDFFirst evidence of predation on the native endangered Iberian desman (Galemys pyrenaicus) and Iberian water shrews (Neomys anomalus) by the invasive species American mink using eDNA tools in Extremadura (Spain).
Mol Biol Rep
December 2024
Genetic and Animal Breeding, Faculty of Veterinary, Universidad de Extremadura, Cáceres, 10071, Spain.
Background: Wildlife conservation and management aims to restore population declines, it is the vulnerable or endangered populations who require the greatest conservation efforts. In this context, non-invasive sampling has been evaluated as an option for reporting prey/predator impact. Galemys pyrenaicus is currently threatened throughout its range, and cohabits with Nemys anomalus, in Extremadura (Spain).
View Article and Find Full Text PDFIdentification and Analysis of Small Nucleolar RNAs by Real-Time Quantitative PCR.
Methods Mol Biol
December 2024
Aging + Cardiovascular Discovery Center, Department of Cardiovascular Sciences, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA.
Article Synopsis
- The Human Genome Project revealed that only about 2% of the human genome codes for proteins, labeling the rest as noncoding or "junk" DNA.
- Over the past two decades, research has shown that noncoding RNAs play crucial roles in regulating cell and tissue functions, classified into long noncoding RNAs and small noncoding RNAs.
- Small nucleolar RNAs (snoRNAs), a type of small noncoding RNA, are vital for modifying other RNAs and are analyzed using techniques like real-time quantitative PCR (RT-qPCR).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!