The imaging time-of-flight secondary-ion-mass-spectrometry (TOF-SIMS) method was utilized to address the problem of cholesterol localization in rat tissues. Rat kidneys were fixed, cryoprotected by sucrose, frozen, sectioned by cryoultramicrotomy, and dried at room temperature. The samples were either covered with a thin silver layer or analyzed uncovered in an imaging TOF-SIMS instrument equipped with an Au1-3(+)-source. The yield of desorbed secondary ions for some species was up to 600-fold higher after silver coating of the samples. Reference samples of cholesterol were silver-coated and analyzed by TOF-SIMS to define significant peaks, specific for cholesterol. Such peaks were found at m/z = 386 (C27H46O+), m/z = 493 (C27H46O107Ag+), m/z = 495 (C27H46O109Ag+), m/z = 879 (C54H92O2 107Ag+), and m/z = 881 (C54H92O2 109Ag+). The silver-cationized cholesterol (493 < or = m/z < or = 495) signal was localized by imaging TOF-SIMS in the kidney sections and showed a high cholesterol content in the kidney glomeruli. A more diffuse distribution of cholesterol was also found over areas representing the cytoplasm or plasma membrane of the epithelial cells in the proximal tubules of rat kidney.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jemt.20133 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of the Micro-Morphology and Compositional Distribution of Chang'e-5 Lunar Soil Mineral Surfaces Using TOF-SIMS.
Adv Sci (Weinh)
January 2025
Experimental Center of Advanced Materials, School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, China.
The lunar soil samples returned by China's Chang'e-5 (CE-5) contain valuable information on geological evolutions on the Moon. Herein, by employing high-resolution time-of-flight secondary ion mass spectrometry (TOF-SIMS), five rock chip samples from the CE-5 lunar soil are characterized in-depth, which reveal micro-morphological and compositional features. From the elemental/molecular ion distribution images, minerals such as pyroxene, ilmenite, feldspar, K-rich glass, silica, and silicate minerals are identified, along with their occurrence states and distribution results.
View Article and Find Full Text PDFToF-SIMS Parallel Imaging MS/MS of Lead Soaps in Embedded Paint Cross Sections.
Anal Chem
January 2025
Maastricht MultiModal Molecular Imaging (M4i) Institute, Maastricht University, Universiteitssingel 50, Maastricht 6229 ER, The Netherlands.
In the field of cultural heritage, and more specifically in oil paintings, the ability to unambiguously identify and locate metal soaps is of great interest for a better understanding of painting degradation. Here, we demonstrate the use of a Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) instrument capable of tandem mass spectrometry imaging for the unambiguous identification and localization of lead soaps in cross sections of samples of old oil paintings at high spatial resolution. It is shown that the specific fragmentation pattern of lead soaps is dictated by the loss of the lead ion and that fragmentation occurs on the hydrocarbon chains of the fatty acids.
View Article and Find Full Text PDFAnalysis of time-of-flight secondary ion mass spectrometry data of human skin treated with diclofenac using sparse autoencoder.
Anal Bioanal Chem
December 2024
Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-Kitamachi, Musashino, Tokyo, 180-8633, Japan.
Methods that facilitate molecular identification and imaging are required to evaluate drug penetration into tissues. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), which has high spatial resolution and allows 3D distribution imaging of organic materials, is suitable for this purpose. However, the complexity of ToF-SIMS data, which includes nonlinear factors, makes interpretation challenging.
View Article and Find Full Text PDFDirect covalent attachment of fluorescent molecules on plasma polymerized nanoparticles: a simplified approach for biomedical applications.
J Mater Chem B
December 2024
School of Physics, University of Sydney, Sydney, NSW 2006, Australia.
Polymeric nanoparticles surface functionalised with fluorescent molecules hold significant potential for advancing diagnostics and therapeutic delivery. Despite their promise, challenges persist in achieving robust attachment of fluorescent molecules for real-time tracking. Weak physical adsorption, pH-dependent electrostatic capture, and hydrophobic interactions often fail to achieve stable attachment of fluorescent markers.
View Article and Find Full Text PDFMachine Learning Correlation of Electron Micrographs and ToF-SIMS for the Analysis of Organic Biomarkers in Mudstone.
J Am Soc Mass Spectrom
January 2025
Department of Earth and Environmental Sciences, University of Illinois Chicago, Chicago, Illinois 60607, United States.
The spatial distribution of organics in geological samples can be used to determine when and how these organics were incorporated into the host rock. Mass spectrometry (MS) imaging can rapidly collect a large amount of data, but ions produced are mixed without discrimination, resulting in complex mass spectra that can be difficult to interpret. Here, we apply unsupervised and supervised machine learning (ML) to help interpret spectra from time-of-flight-secondary ion mass spectrometry (ToF-SIMS) of an organic-carbon-rich mudstone of the Middle Jurassic of England (UK).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!