[Effect of Yigu capsule on NO and NOS of rat osteoblasts in vitro].

Objective: The effects of Yigu Capsule on NO and NOS of osteoblasts were studied.

Methods: (1) Forty 12-month old Sprague-daweley female rats were divided into randomly four groups (Yigu Capsule high dose group, medium dose group and low dose group; saline group), the drug-containing serum and control serum were prepared. (2) The new-born Sprague-daweley rat Osteoblasts were cultured with different Yigu Capsule drug-containing serum concentration and at different exposure time. The mRNA levels of eNOS and iNOS were measured by RT-PCR; the activities of eNOS and iNOS were measured by test kit at different exposure time. NO secretion of osteoblasts was observed, too.

Results: The mRNA levels and the activities of eNOS and iNOS and NO secretion of osteoblasts were higher than the control groups respectively after 48,72 and 96 hours (P < 0.01 or P <0.05).

Conclusions: Yigu Capsule drug-containing serum can promote NOS mRNA and NOS activities expressions and NO secretion of osteoblasts. These results may be parts of the mechanism of Yigu Capsule preventing and treating osteoporosis.