AI Article Synopsis
- Developed seven specific DNA markers for the Q chromosome in tobacco (Nicotiana tabacum) using RAPD and ISSR techniques.
- Identified tobacco monosomic plants from F1 progeny through cross-breeding, showcasing the effectiveness of these markers.
- Discussed the significance of the Q chromosome in embryo sac development and proposed a novel method for identifying DNA markers for specific chromosomes in the Nicotiana genus.
Article Abstract
We developed seven Q-chromosome-specific DNA markers in Nicotiana tabacum by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis using two hybrid lines, and we were able to identify tobacco monosomic plants among F1 progeny derived from the cross N. tabacum Haplo-QxN. tabacum cv. Samsun NN using Q-chromosome-specific DNA markers. Based on the results, we discuss the roles of the Q chromosome in embryo sac development and embryogenesis. Here, we propose a new method for identifying DNA markers for a particular chromosome in the genus Nicotiana.
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| http://dx.doi.org/10.1093/pcp/pch204 | DOI Listing |
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