Escherichia coli O157 isolates from bovine hide (n=117) and beef trimmings (n=32) from a single abattoir were examined by pulsed field gel electrophoresis (PFGE). Using BioNumerics software, dendrograms of isolates from each sample type (i.e. hide and beef trimming) were produced. In assessing the genetic relatedness of isolates, a similarity criterion of 80% was applied. The 117 E. coli O157 hide isolates were grouped into 14 clusters, comprising of 109 different PFGE profiles. Of the 109 different PFGE profiles, 8 were common to multiple isolates (i.e. shared 100% similarity by PFGE). The 32 E. coli O157 beef trimming isolates produced 28 different PFGE profiles and 2 clusters. Of the 28 PFGE profiles, 2 were common to multiple isolates and the remaining 26 were distinct. On a number of sampling occasions, isolates displaying identical PFGE patterns were recovered from multiple isolates collected from a single sample type (i.e. hides or trimmings), suggesting cross contamination from contaminated hides/animals to uncontaminated hides/animals and from contaminated beef trimmings to uncontaminated beef trimmings during abattoir operations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2004.10.014 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Expression of concern: Electrochemical genosensor based on gold nanostars for the detection of O157:H7 DNA.
Anal Methods
January 2025
Physics and Electronics, Department of Science and Technology, Linköping University, SE-601 74 Norrköping, Sweden.
Expression of concern for 'Electrochemical genosensor based on gold nanostars for the detection of O157:H7 DNA' by Nasrin Razmi , , 2022, , 1562-1570, https://doi.org/10.1039/D2AY00056C.
View Article and Find Full Text PDFMicrobial seafood safety assessment following a marine mucilage disaster in the Sea of Marmara.
Environ Microbiol Rep
February 2025
Faculty of Engineering, Department of Chemistry, Istanbul University-Cerrahpaşa, Istanbul, Türkiye.
Marine mucilage disasters, primarily caused by global warming and marine pollution, threaten food security and the sustainability of marine food resources. This study assessed the microbial risks to public health in common sole, deep-water rose shrimp, European anchovy, Atlantic horse mackerel and Mediterranean mussel following the mucilage disaster in the Sea of Marmara in 2021. The total viable count, total Enterobacteriaceae count and the presence of Escherichia coli O157:H7, Salmonella spp.
View Article and Find Full Text PDFGenetic profiling of extended-spectrum β-Lactamase and carbapenemase-producing Escherichia coli O157:H7 from clinical samples among diarrheal patients in Shashemene, Ethiopia.
BMC Infect Dis
January 2025
Department of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Background: Escherichia coli (E. coli) O157:H7, associated with diarrhea, poses a global health risk. In Ethiopia, where diarrhea is common, there is limited knowledge about these resistant strains and a lack of data on Extended-Spectrum β-Lactamase (ESBL) and carbapenemase production.
View Article and Find Full Text PDFAn efficient, high-throughput enrichment system for the rapid detection of E. coli at low concentrations in water.
Anal Chim Acta
February 2025
School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China; University of Chinese Academy of Sciences, Beijing, 100049, China; State Key Laboratory of Applied Optics, Changchun, 130033, China; Key Laboratory of Advanced Manufacturing for Optical Systems, Chinese Academy of Sciences, Changchun, 130033, China. Electronic address:
Certain virulent strains of Escherichia coli (E. coli), notably the enterohemorrhagic serotype O157:H7, are recognized for causing diarrhea, gastroenteritis, and a range of illnesses that pose significant risks to public health and the safety of drinking water supplies. Early detection and management of E.
View Article and Find Full Text PDFVirulent Bacteriophages for Controlling Shiga Toxin-Producing Escherichia coli (STEC) Without Inducing Toxin Production.
J Infect Dis
January 2025
Division of Environmental Health Sciences, School of Public Health, University of Minnesota, St. Paul, MN 55108, USA.
Shiga toxin-producing Escherichia coli (STEC) infections pose a significant public health challenge, characterized by severe complications including hemolytic uremic syndrome (HUS) due to Shiga toxin (Stx) production. Current therapeutic approaches encounter a critical limitation, as conventional antibiotic treatment is contraindicated due to its propensity to trigger bacterial SOS response and subsequently enhance Stx production, which increases the likelihood of developing HUS in antibiotic-treated patients. The lack of effective, safe therapeutic options has created an urgent need for alternative treatment strategies for STEC infections.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!