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The rat bladder umbrella cell keratin network: Organization, dependence on the plectin cytolinker, and responses to bladder filling.
Mol Biol Cell
November 2024
Department of Medicine Renal-Electrolyte Division and George M. O'Brien Pittsburgh Center for Kidney Research, University of Pittsburgh School of Medicine, Pittsburgh, PA.
The keratin cytoskeleton and associated desmosomes contribute to the mechanical stability of epithelial tissues, but their organization in native bladder umbrella cells and their responses to bladder filling are poorly understood. Using whole rat bladders in conjunction with confocal microscopy, super-resolution image processing, three-dimensional image reconstruction, and platinum replica electron microscopy, we identified a cortical cytoskeleton network in umbrella cells that was organized as a dense tile-like mesh comprised of tesserae bordered by cortical actin filaments, filled with keratin filaments, and cross-linked by plectin. Below these tesserae, keratin formed a subapical meshwork and at the cell periphery a band of keratin was linked via plectin to the junction-associated actin ring.
View Article and Find Full Text PDFCancer cell extravasation requires plectin-mediated delivery of MT1-MMP at invadopodia.
Br J Cancer
September 2024
Biological Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada.
Article Synopsis
- The study investigates how MT1-MMP, a protease involved in cancer cell movement, is recruited to invadopodia in metastatic breast cancer cells, focusing on its interaction with a protein called plectin.
- Through techniques like mass spectrometry and siRNA, researchers found that plectin is crucial for forming invadopodia and facilitating the enzyme's delivery, significantly influencing cancer cell invasiveness.
- Targeting the MT1-MMP and plectin interaction might pave the way for new therapies aimed at reducing cancer metastasis.
Homozygosity for a Rare Variant Suggests a Contributory Role in Congenital Insensitivity to Pain.
Int J Mol Sci
June 2024
National Biobank of Thailand, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani 12120, Thailand.
Congenital insensitivity to pain is a rare human condition in which affected individuals do not experience pain throughout their lives. This study aimed to identify the molecular etiology of congenital insensitivity to pain in two Thai patients. Clinical, radiographic, histopathologic, immunohistochemical, and molecular studies were performed.
View Article and Find Full Text PDFThe umbrella cell keratin network: organization as a tile-like mesh, formation of a girded layer in response to bladder filling, and dependence on the plectin cytolinker.
bioRxiv
June 2024
Department of Medicine Renal-Electrolyte Division and George M. O'Brien Pittsburgh Center for Kidney Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
The keratin cytoskeleton and associated desmosomes contribute to the mechanical stability of epithelial tissues, but their organization in bladder umbrella cells and their responses to bladder filling are poorly understood. Using super-resolution confocal microscopy, along with 3D image reconstruction and platinum replica electron microscopy, we observed that the apical keratin network of umbrella cells was organized as a dense tile-like mesh comprised of tesserae bordered on their edges by cortical actin filaments, filled with woven keratin filaments, and crosslinked by plectin. A band of keratin was also observed at the cell periphery that was linked to the junction-associated actin ring by plectin.
View Article and Find Full Text PDFPlectin Deficiency in Fibroblasts Deranges Intermediate Filament and Organelle Morphology, Migration, and Adhesion.
J Invest Dermatol
March 2024
Department of Cell and Developmental Biology, Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria. Electronic address:
Plectin, a highly versatile and multifunctional cytolinker, has been implicated in several multisystemic disorders. Most sequence variations in the human plectin gene (PLEC) cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD), an autosomal recessive skin-blistering disorder associated with progressive muscle weakness. In this study, we performed a comprehensive cell biological analysis of dermal fibroblasts from three different patients with EBS-MD, where PLEC expression analyses revealed preserved mRNA levels in all cases, whereas full-length plectin protein content was significantly reduced or completely absent.
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