Objective: To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction.
Methods: HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm.
Results: The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%).
Conclusion: The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.
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http://dx.doi.org/10.3736/jcim20050116 | DOI Listing |
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