AI Article Synopsis
- Isolating high-quality DNA from plants like Actinidia is challenging due to their high levels of polyphenolics and polysaccharose.
- The paper presents a modified CTAB method that successfully isolates high-quality genomic DNA from these plants.
- The extracted DNA is suitable for Southern blotting and other DNA research, and the protocol allows for both quick and macro-DNA extraction.
Article Abstract
It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.
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