According to the 5' and 3' untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3, a pair of primers was designed employing computer analysis. With the primers,polymerase chain reaction (PCR) was performed and a 1080 bp fragment was obtained from BmNPV strain Shandong. Sequencing result showed that it was p35 gene (GenBank accession number: AY157746), and it included an open reading frame of 900 bp, encoding 299 amino acids with Mr=34.91 kDa and pI=6.40. BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Genotype-phenotype associations in individuals with Diamond Blackfan anaemia.
EJHaem
December 2024
Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics National Cancer Institute, NIH, Rockville Bethesda Maryland USA.
Introduction: Diamond Blackfan anaemia (DBA) is a rare disorder characterized by failure of red blood cell production, congenital abnormalities and cancer predisposition, primarily caused by pathogenic germline variants in genes encoding ribosomal proteins.
Methods: We conducted a genotype-phenotype and outcome study of 121 patients with DBA spanning the 20-year history of the National Cancer Institute's Inherited Bone Marrow Failure Syndromes study. Patient phenotypes were compared by large versus small ribosomal protein genes, across genes with >5 cases (, , and ) and by type of pathogenic variants (hypomorphic versus null, large deletions versus others).
Glucose-Sensing ChREBP Protein in the Pathogenesis of Dia-betic Retinopathy.
bioRxiv
December 2024
Wake Forest University, School of Medicine, Department of Biochemistry.
Glucose-sensing ChREBP and MondoA are transcriptional factors involved in lipogenic, inflammatory, and insulin signaling pathways implicated in metabolic disorders; however, limited ocular studies have been conducted on these proteins. We aimed to investigate the potential role of ChREBP in pathogenesis of diabetic retinopathy (DR). We used diabetic human and mouse retinal cryosections analyzed by immunohistochemistry.
View Article and Find Full Text PDFPI3K/Akt inhibition promotes AR activity and prostate cancer cell proliferation through p35-CDK5 modulation.
Biochim Biophys Acta Mol Basis Dis
February 2025
Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan. Electronic address:
Article Synopsis
- Aberrant activation of the PI3K/Akt pathway is associated with the severity of prostate cancer (PCa), while the androgen receptor (AR) plays a vital role in the early stages of PCa development.
- A study found that p35-CDK5 acts as a mediator between PI3K/Akt and AR, promoting PCa cell growth and stability of the androgen receptor, particularly when the PI3K/Akt signaling is inhibited.
- Inhibiting PI3K/Akt not only increases p35 expression and enhances AR activation in PCa cells but also suggests that p35-CDK5 is essential for cell survival when PI3K/Akt activity is blocked.
The discovery of the RCC1::IRF4 Fusion in CLL patients with t(1;6)(p35.3;p25.2) chromosomal translocation.
Br J Haematol
December 2024
Department of Hematology, Medical University of Lodz, Lodz, Poland.
Article Synopsis
- Jayne et al. reveal the molecular details of a specific chromosomal translocation (t(1;6)(p35.3;p25.2)) found in chronic lymphocytic leukaemia patients.
- This translocation involves the IRF4 gene on chromosome 6 and the RCC1 gene on chromosome 1, indicating a fusion between RCC1 and IRF4.
- The study suggests that this chromosomal abnormality could have significant prognostic implications for patients with this type of leukaemia.
The chromosomal translocation t(1;6)(p35.3;p25.2), recurrent in chronic lymphocytic leukaemia, leads to RCC1::IRF4 fusion.
Br J Haematol
December 2024
The Ernest and Helen Scott Haematological Research Institute, Leicester Cancer Research Centre, University of Leicester, Leicester, UK.
Article Synopsis
- * A study of 10 cases revealed that this translocation connects the interferon regulatory factor 4 (IRF4) gene on chromosome 6 with the regulator of chromosome condensation 1 (RCC1) gene on chromosome 1, resulting in fusion transcripts.
- * Despite the fusion, the expression levels of RCC1 and IRF4 proteins remained normal, and the cases also displayed typical mutations related to CLL, suggesting a linkage with the IGHV-unmutated subtype.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!