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[Advances in allelic polymorphism at class II of the pig MHC(SLA)]. | LitMetric

AI Article Synopsis

  • The article discusses the regional mapping, molecular structure, genotyping, and polymorphism of SLA class II genes in pigs, focusing on their major histocompatibility complex (MHC).
  • It highlights the variability in allelic polymorphism of these genes and describes a method using locus-specific oligonucleotide primers and RFLP analysis for efficient genotyping from genomic DNA.
  • The study also reveals that SLA class II polymorphism is linked to the antigenic peptide binding sites, with sequence analysis identifying four GC-rich regions in the exon 2 of SLA-DQB and SLA-DRB1 genes.

Article Abstract

This article gave a detailed introduction about regional map,molecular structure,genotyping and polymorphism of SLA class II genes. The pig major histocompatibility complex (MHC) class II antigens have been known to exhibit a different degree of allelic polymorphism. The locus-specific oligonucleotide primers and RFLP analysis provide a simple and rapid method for genotyping expressed SLA class II from genomic DNA. SLA class II polymorphism was related to the antigenic peptide binding sites. Detailed analysis of sequences showed that there were 4 GC-rich sequences in exon 2 of SLA-DQB and SLA-DRB1 genes.

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