AI Article Synopsis
- cDNA-AFLP is used to identify genes that are expressed differently in two wheat lines with varying resistance to salt (NaCl) from the same seed.
- A total of cDNA fragments associated with salt tolerance were identified, including one called SIR73, which has a 32% similarity to human transcription factors.
- Northern analysis shows that SIR73 is significantly induced by NaCl stress, with higher expression in the salt-resistant line (SR) compared to the salt-sensitive line (SS), suggesting its role in regulating gene expression during salt stress.
Article Abstract
cDNA-AFLP (amplified fragment length polymorphism) is used to isolate genes differentially expressed in two wheat lines with the different resistance to NaCl derived from a single seed. A lot of cDNA fragments related to salt tolerance are obtained. Of with the number 73 cDNA fragment encodes for a transcription factors with an 32% similarity to human transcription factors in the relative amino acid which is named SIR73. Northern analysis confirms that SIR73 is strongly induced by NaCl stress and the expression in SR is more strongly induced than in SS.SIR73 may be involved in the regulation of gene expression in salt stress in wheat.
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