Transgenic plant as bioreactor has been used to produce recombinant proteins for medicinal purposes, including mammalian antibodies, blood substitutes and vaccines. As the demand for biopharmaceuticals is expected to increase, transgenic plants have the potential to provide virtually unlimited quantities of proteins for use as tools in both human health care and the bioscience. This paper reviews the recent developments in this field and the prospect of commercial applications.
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Nat Commun
January 2025
School of Biomedical Engineering, Tsinghua Medicine; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Cultured meat needs edible bio-scaffolds that provide not only a growth milieu for muscle and adipose cells, but also biomimetic stiffness and tissue-sculpting topography. Current meat-engineering technologies struggle to achieve scalable cell production, efficient cell differentiation, and tissue maturation in one single culture system. Here we propose an autoclaving strategy to transform common vegetables into muscle- and adipose-engineering scaffolds, without undergoing conventional plant decellularization.
View Article and Find Full Text PDFJ Fungi (Basel)
December 2024
Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Hyaluronidases have been a subject of great interest in medical and cosmeceutical applications. Previously, our group demonstrated that the venom glands of contain hyaluronidase enzymes (VesT2s), and heterologous expression of the corresponding gene () in systems results in inclusion bodies, necessitating functional folding using urea. Here, we report the successful heterologous expression of VesT2a in the expression system, with gene construction achieved using Golden.
View Article and Find Full Text PDFChemosphere
December 2024
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
This study evaluated the integration of electrocoagulation into a lab-scale membrane bioreactor (EC-MBR) for treating wastewater from a detergent manufacturing plant. The EC-MBR system achieved a higher chemical oxygen demand (COD) and anionic surfactant removal efficiencies of 95.1% and 99.
View Article and Find Full Text PDFHuan Jing Ke Xue
January 2025
National Engineering Laboratory of Urban Sewage Advanced Treatment and Resource Utilization Technology, Faculty of Architecture, Civil and Transportation Engineering, Beijing University of Technology, Beijing 100124, China.
To achieve non-carbon dioxide greenhouse gas emission reduction and control in municipal wastewater treatment plants (WWTPs), this study conducted one-year long-term monitoring of nitrous oxide (NO) in the anaerobic-anoxic-aerobic (AO) process of a large-scale municipal wastewater treatment plant in Beijing. The experimental results showed that the anaerobic and anoxic zones of the AO process could effectively remove dissolved NO contained in the return sludge, while the aerobic zone was the main area for NO generation and emission, and its generation pathway may have been dominated by ammonia oxidizing bacteria (AOB) denitrification. A significant difference was observed between winter and summer NO production, and the difference in the average NO release flux was up to 7.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
December 2024
Fudan University, School of Pharmacy, CHINA.
Drimane-type merosesquiterpenoids (DMT) are a class of natural products with diverse structures and broad biological activity. Classical DMT synthesis relies on atom-inefficient plant-derived chiral pool building blocks, while alternative drimane-type building blocks such as drimenol and albicanol offer more direct routes but face production challenges. In this study, we engineered a microbial platform for efficient production of these building blocks.
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