AI Article Synopsis
- The study analyzed the chemical content and enzyme activities in homogenized rabbit livers, using techniques like centrifugation to separate components.
- It measured various proteins and vitamins, finding that certain substances like protein and vitamin A were evenly distributed, while others like vitamin C were concentrated in the supernatant.
- The findings suggest that to accurately understand biochemical changes, evaluations need to include all parts—the whole homogenate, supernatant, and sediment.
Article Abstract
Evaluation was performed of chemical compound contents and enzyme activities in the whole homogenate, its supernatant and sediment. Six rabbit livers were pulverized in liquid nitrogen and homogenized. After centrifugation, the contents of protein, haemoglobin, vitamin A, vitamin E, vitamin C, as well as the activities of cathepsin B, cathepsin D, superoxide dismutase, catalase, glutathione peroxidase and reductase were assessed in the whole homogenate, its supernatant and sediment. Protein, vitamin A, superoxide dismutase, catalase, cathepsin D, glutathione peroxidase and reductase reveal uniform localisation. Vitamin C and cathepsin B are localized in supernatant, whereas haemoglobin is localized mainly in sediment. Evaluation of chemical compounds and enzyme activities should be performed in the whole homogenate, supernatant and sediment to obtain a real interpretation of biochemical disturbances in the investigated material.
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