Intraocular levels of methotrexate after oral low-dose treatment in chronic uveitis.

Purpose: To determine the intraocular levels of methotrexate in low-dose treatment of noninfectious uveitis.

Methods: One day after oral administration, the methotrexate level was measured in the aqueous humor and serum of a patient with noninfectious uveitis, who underwent cataract surgery. A fluorescence polarization immunoassay was used for determination.

Results: After oral administration, methotrexate was only measurable in aqueous humor but not in serum.

Conclusion: In uveitis, orally administered low-dose methotrexate reaches detectable levels in aqueous humor, even in the absence of detectable levels in serum.