[Study on expression of adherent proteins related to regulation of hematopoiesis in long-term cultured human bone marrow stromal cells].

Objective: To study the molecular basis of bone marrow stromal cell supporting hematopoiesis.

Methods: Immunoelectron microscopic localization of adherent protein related to regulation of hematopoiesis was performed by immunocolloidal gold labelling technique in the adherent layer of long-term cultured human bone marrow stromal cells (LTCHBMSC).

Results: Adherent proteins were found to be expressed on LTCHBMSC. Double labelling revealed co-expression of these proteins and some specific markers for endothelial cells (vWF) and fibroblasts (Fb-sp). It was suggested that endothelial cells and fibroblasts in LTCHBMSC could synthesize and secrete adherent proteins, which bound to the surface of stromal cells and extracellular matrix.

Conclusion: Adherent proteins and all kinds of hematopoietic growth factors secreted by stromal cells constitute a complex network supporting hematopoiesis.