AI Article Synopsis
Article Abstract
This study compared the effects of activation treatments on the development and ploidy of nuclear transferred (NT) pig embryos. After in vitro maturation of oocytes collected from the slaughterhouse, oocytes were enucleated and reconstructed by transfer of donor cells and fusion with three DC pulses (1.4 kV/cm, 20 musec). Oocytes were pulsed thrice electrically with 1.4 kV/cm for 20 musec and NT eggs were divided into three treatment groups: Group 1 (no further treatment), Group 2 (10 mug/mL cycloheximide [CHX], 3 hr), and Group 3 (1.9 mM 6-dimethylaminopurine [DMAP], 3 hr). All the eggs were cultured in sets of 30 in 60 muL drops of NCSU-23 supplemented with 4 mg/mL fatty acid free BSA, and compared for the rates of development and ploidy. The rates of cleavage, development, and total cell number of parthenotes in Group 3 were significantly (P < 0.05) higher than those in Groups 1 and 2. Cleavage rates of NT embryos in Group 1 were significantly (P < 0.05) lower than those in Groups 2 and 3 (73% vs. 81% and 82%, respectively). Development into blastocyst stage and total cell number of NT embryos in Group 3 were significantly (P < 0.05) higher than those in Groups 1 and 2. Although the embryos in Group 3 had higher development, approximately 58% of NT embryos evaluated were abnormal ploidy (6% haploidy, 9% polyploidy, and 42% mixoploid). The results suggested that although DMAP enhanced development and higher cell number, due attention should be paid to abnormal ploidy in pig NT embryos.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mrd.20211 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Expression Pattern and Functional Analysis of Extracellular Vesicle Long Non-Coding RNAs from Uterine Fluid During Implantation in Pig.
Animals (Basel)
January 2025
State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Long non-coding RNAs (lncRNAs) act as competing endogenous RNAs and play significant roles in porcine embryo development. Extracellular vesicles (EVs) in the uterine fluid (UF) can target and deliver maternal endometrial signalling molecules to embryonic trophoblast cells, exerting crucial regulatory effects during embryo implantation. However, the specific roles of lncRNAs carried by UF-EVs during the embryo implantation period have not been thoroughly reported in the literature.
View Article and Find Full Text PDFLipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum.
Front Mol Biosci
January 2025
Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Introduction: Chronic inflammation caused by infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ's function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.
View Article and Find Full Text PDFApplication of Multiple Base-Editing Mediated by Polycistronic tRNA-gRNA-Processing System in Pig Cells.
Biotechnol Bioeng
January 2025
Key Laboratory of Animal Embryo Engineering and Molecular Breeding of Hubei Province, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan, China.
Gene edited pigs have extensive and important application value in the fields of agriculture and biomedicine. With the increasing demand in medical research and agricultural markets, more and more application scenarios require gene edited pigs to possess two or even more advantageous phenotypes simultaneously. The current production of multi gene edited pigs is inefficient, time-consuming, and costly, and there is an urgent need to develop efficient and accurate multi gene editing application technologies.
View Article and Find Full Text PDFPorcine endometrial epithelial organoids: Generation, characterization, and the impact of stromal cells.
Theriogenology
January 2025
Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, College of Life Science, Northeast Agricultural University, Harbin, 150030, China. Electronic address:
Endometrial organoids (EOs) are three-dimensional models that emulate the endometrium, serving as an invaluable in vitro tool for investigating the cellular and molecular mechanisms underlying endometrial physiology and pathology during the estrous cycle and pregnancy. While significant progress has been made in the establishment and optimization of EOs for both humans and mice, research on such models in other species remains limited. This study aimed to develop porcine endometrial epithelial organoids (EEOs) to explore the regulatory mechanisms of uterine function and maternal-fetal interactions during porcine pregnancy, which are critical for enhancing reproductive efficiency and improving embryo transfer techniques.
View Article and Find Full Text PDFEquol Alleviates the In Vitro Aging-Induced Disruption of Porcine Oocytes.
Reprod Domest Anim
January 2025
College of Animal Science & Technology, Guangxi University, Nanning, Guangxi, China.
Oocyte quality is crucial for determining the subsequent embryo developmental capacity and reproductive outcomes. However, aging is detrimental to oocyte quality. Previous studies have demonstrated that soy isoflavones have positive effects on the reproductive performance of female pigs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!