To clarify the relationships between soil microorganisms and soil fertility under the condition of long-term fertilization, a 12-year fertilization experiment was installed on a fluvo-aquic soil, and the amount of soil microorganisms and the content of soil nutrients were determined and analyzed. The results showed that chemical fertilizers combined with organic manure could significantly improve soil fertility and increase the amount of soil microorganisms. Organic manure was obviously superior to corn straw in improving soil fertility. The correlation between soil microorganisms and soil fertility was significant. A positive correlation was found between the amount of bacteria and azotobacteria and the contents of organic matter, total N, alkalified N, total P and available P. The relationship between the amount of fungi and actinomycetes and the content of soil nutrients was not obvious.
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