Objective: To study the effect of several factors on the quantity of hypericin in H. perforatum callus.
Method: High efficiency liquid phase chromatography and plant tissue culture were applied.
Result And Conclusion: When the ratio of nitro-nitrogen to amina-nitrogen is 3:1, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Localization of Secondary Metabolites in Relict Gymnosperms of the Genus In Vivo and in Cell Cultures In Vitro, and the Biological Activity of Their Extracts.
Life (Basel)
December 2024
Department of Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street 49, Moscow 127434, Russia.
In order to scientifically search for new sources of secondary metabolites with valuable qualities for phytopharmacognosy, tasks requiring a step-by-step solution were set. The primary task is the development of technologies for obtaining in vitro highly productive biomass of cells of relict gymnosperms of the genus , capable of accumulating various classes of secondary metabolites. The study of the accumulation and localization of secondary metabolites allowed us to evaluate the biological activity and cytotoxicity of in vitro cultures.
View Article and Find Full Text PDFEvaluation of Cytotoxic Activity of Cell Biomass from and on Melanoma Cancer Cell.
Molecules
October 2024
Laboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Diseases Prophylaxis, Poznan University of Medical Sciences, Collegium Pharmaceuticum, 3 Rokietnicka St., 60-806 Poznan, Poland.
Article Synopsis
- - Melanoma, a type of skin cancer, is becoming more common, and there is interest in using plant-based products that have low toxicity as potential treatments.
- - The study focused on inducing callus biomass from specific plants using growth regulators and creating methanolic extracts to test their effects on human melanoma (MeWo) cells and human fibroblast cells (MRC-5).
- - The results showed that certain extracts and fractions had significant cytotoxic effects on melanoma cells, indicating their potential for use in cancer treatment, especially the extracts with higher concentrations of methanol.
CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.
Funct Integr Genomics
November 2024
Mountain Research Center for Field Crop, SKUAST Srinagar, Jammu and Kashmir, Khudwani, India.
The objective of present review is to provide a scientific overview of sugarcane as a potential feedstock for biofuel and use of genome editing approach for improvement of industrial and agronomical traits in sugarcane. Sugarcane, a perennial tropical grass with a high biomass index, is a promising feedstock for bioethanol production, and its bagasse, rich in lignocellulosic material, serves as an ideal feedstock for producing second-generation bioethanol. To improve the conversion of sugarcane biomass into biofuels, developing varieties with improved biomass degradability and high biomass and sucrose content is essential.
View Article and Find Full Text PDFTargeted regulation of 5-aminolevulinic acid enhances flavonoids, anthocyanins and proanthocyanidins accumulation in Vitis davidii callus.
BMC Plant Biol
October 2024
College of Horticulture, Fujian Agriculture and Forestry University, Shangxiadian Road 15, Cangshan District, Fuzhou, Fujian, 350002, P.R. China.
Background: Spine grape (Vitis davidii) is a promising source of high-quality anthocyanins, with vast potential for application in food, pharmaceutical, and cosmetic industries. However, their availability is limited by resource constraints. Plant cell culture has emerged as a valuable approach for anthocyanin production and serves as an ideal model to investigate the regulation of anthocyanin biosynthesis.
View Article and Find Full Text PDFGreen synthesized iron oxide nanoparticles as a potential regulator of callus growth, plant physiology, antioxidative and microbial contamination in Oryza sativa L.
BMC Plant Biol
October 2024
Depatment of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Article Synopsis
- - The study focuses on improving plantlet regeneration in tissue culture by utilizing iron oxide nanoparticles (FeO-NPs) as a nutrient source in Murashige and Skoog (MS) media to enhance callus formation and control contamination.
- - The results showed that FeO-NPs significantly increased callus induction and regeneration rates compared to regular MS media, with notable improvements in callus weight, diameter, and root/shoot numbers, indicating a more effective growth environment.
- - Applying FeO-NPs as a foliar spray led to substantial increases in biomass, height, and chlorophyll content in two rice varieties, while also enhancing enzymatic activities that help plants respond to stress, suggesting that this approach could be beneficial for
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!