Unlabelled: To clarify whether nicotine affected the early embryo development, the paper investigated the influence of nicotine on embryonic stem (ES) cells specific gene Oct-4.
Methods: ES cells were treated with nicotine (1-1000 nM) and/or 10 microM tubocurarine (a usual nicotinic acetylcholine receptors [nAChRs] blocker) for 24 h. Subsequently, they were purified to discard feeder cells and total RNA was isolated. Fgf-5 was amplified to detect the purification of ES cells, and the relative expression of Oct-4 and beta-actin to GAPDH was analyzed with RT-PCR.
Results: RT-PCR analysis illustrated that nicotine (10-1000 nM) significantly enhanced Oct-4 transcription, while had no effect on beta-actin transcription; meanwhile, compared with nicotine (100 nM and 1000 nM) treatment alone, tubocurarine inhibited Oct-4 transcription evidently.
Conclusion: It is reasonable to assume that nicotine could influence the development and differentiation of ES cells, and impinge on the early embryo development.
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