AI Article Synopsis
- Linker histone H1B was identified as a coeluting protein with antiviral activity during the purification of the HIV-1 resistance factor (HRF) from cell supernatants.
- Analysis showed that the presence of H1B correlated with HIV-1 resistance, as silencing H1B significantly reduced the antiviral activity of HRF(+) cell supernatants.
- The study suggests that ubiquitinated H1B may act as a cofactor for HRF, influencing its expression and secretion from CD4(+) T cells, which becomes crucial in resisting HIV-1 infection.
Article Abstract
Linker histone H1B (H1B) coeluted with an antiviral activity during the purification of HIV-1 resistance factor (HRF) from supernatants of HRF(+) cells. Western blot analysis of the supernatant using alpha-H1 and alpha-ubiquitin antibodies detected the same band of roughly 46 kDa; this band was absent from the control supernatant. Depletion of histone from biologically active material did not affect its potential, suggesting that ubiquitinated H1B is not required for the HRF-mediated antiviral protection in HIV-1 susceptible target cells; however, specific silencing of histone H1B via RNAi in HRF(+) cells reduced the biological activity of cell culture supernatants by 96% and reversed the HIV-1 resistance phenotype of HRF(+) cells. Exposure to HRF induced ubiquitination and secretion of H1B from target HIV-1 susceptible cells, suggesting that ubiquitinated H1B is a cofactor of HRF, possibly regulating its expression and secretion from CD4(+)T cells induced to resist HIV-1 infection.
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| http://dx.doi.org/10.1021/bi0492758 | DOI Listing |
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