The humic substances extracted from different soil depths are separated into humic (HA) and fulvic (FA) acids, and characterized for their chemical composition, proton exchange capacity, spectroscopic characteristics and binding properties to the europium ion. The chemical and spectroscopic results show that FA compared to HA has a relatively high O/C ratio, high acidic functional group contents and low aromatic contents. The synchronous fluorescence spectroscopic results show that the stability constant (K) of the soil humic substances with Eu(III) ion tends to increase as the soil depth becomes deeper, and HA has a slightly stronger binding ability than FA. The measured total site concentrations (C(L)) reveal that Eu(III) ion is loaded onto HA by 62-77% of the total acid sites, but FA is only approximately 50% covered by Eu(III) ion. Information could be useful in understanding the migration of radionuclides in soil layer.
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