A simple one-step purification method, using expanded bed, ion-exchange chromatography, for the fractionation of nisin Z produced by Lactococcus lactis subsp. lactis A164 was developed. The highest dynamic binding capacity (0.92) of the adsorbent was obtained at a superficial velocity of 367 cm h(-1), resulting in approx. 2.7-fold bed expansion. The range of pH for the maximum adsorption was 3-4. The isocratic elution with 0.15 M NaCl led to approx. >90% recovery. Single-step purification of nisin Z from unclarified A164 culture broth resulted in 31-fold purification with a 90% yield.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1023/B:BILE.0000045630.29494.45 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cellulose nanofiber-reinforced antimicrobial and antioxidant multifunctional hydrogel with self-healing, adhesion for enhanced wound healing.
Carbohydr Polym
March 2025
College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper Development, Key Laboratory of Paper Based Functional Materials of China National Light Industry, National Demonstration Center for Experimental Light Chemistry Engineering Education, Xi'an 710021, China.
Current conventional wound dressings used for wound healing are often characterized by restricted bioactivity and devoid of multifunctionality resulting in suboptimal treatment and prolonged healing. Despite recent advances, the simultaneous incorporation of excellent flexibility, good mechanical performance, self-healing, bioactivity, and adhesion properties into the dressings without complicating their efficacy while maintaining simple synthesis remains a grand challenge. Herein, we effectively synthesized hybrid hydrogels of cellulose nanofiber (CNF), polyvinyl alcohol (PVA), and curcumin-modified silver nanoparticles (cAg) through a one-step synthesis method based on hydrogen bonds, dynamic boronic ester bonds, and coordinate covalent bonds.
View Article and Find Full Text PDFOne-step fabrication of ultrathin porous Janus membrane within seconds for waterproof and breathable electronic skin.
Sci Bull (Beijing)
December 2024
State Key Laboratory of Advanced Fiber Materials, Institute of Functional Materials, College of Materials Science and Engineering, Research Base of Textile Materials for Flexible Electronics and Biomedical Applications (China Textile Engineering Society), Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, Shanghai Key Laboratory of Lightweight Composite, Donghua University, Shanghai 201620, China. Electronic address:
It remains a challenge for a simple and scalable method to fabricate ultrathin porous Janus membranes for stretchable on-skin electronics. Here, we propose a one-step droplet spreading phase separation strategy to prepare an ultrathin and easily collected Janus thermoplastic polyurethane (TPU) membrane within seconds. The metal-ion solvation structure mitigated migration kinetics to delay TPU solution demixing, promoting the further penetration of the coagulating solvent.
View Article and Find Full Text PDFConcurrent targeted delivery of doxorubicin and curcumin to the cancer cells using simple and versatile ligand-installed multifaceted chitosan-based nanoconjugates.
J Mater Chem B
January 2025
Amity Institute of Biotechnology, Amity University, Kolkata, West Bengal, 700135, India.
Existing chemotherapeutic approaches against refractory cancers are ineffective due to off-target effects, inefficient delivery, and inadequate accumulation of anticancer drugs at the tumor site, which causes limited efficiency of drug treatment and toxicity to neighboring healthy cells. The development of nano-based drug delivery systems (DDSs) with the goal of delivering desired therapeutic doses to the diseased cells and has already proven to be a promising strategy to address these challenges. Our study focuses on achieving an efficient tumor-targeted delivery of a combination of drugs for therapeutic benefits by developing a versatile DDS by following a simple one-step chemical approach.
View Article and Find Full Text PDFEnhanced Macrophage Uptake of Spray-Dried Phosphatidylserine-Loaded Microparticles for Pulmonary Drug Delivery Applications.
J Drug Deliv Sci Technol
February 2025
Department of Chemical Engineering, University of Rhode Island, Kingston, RI 02881 USA.
Macrophages are an integral part of the innate immune system and act as a first line of defense to pathogens; however, macrophages can be reservoirs for pathogens to hide and replicate. Tuberculosis, influenza virus, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are common diseases whose pathogens are uptaken into macrophages. Current treatments for diseases such as these are limited by the therapeutic delivery method, which typically involves systemic delivery in large, frequent doses.
View Article and Find Full Text PDFPolyvinyl alcohol/chitosan hydrogel based on deep eutectic solvent for promoting methicillin-resistant Staphylococcus aureus-infected wound healing.
Int J Biol Macromol
January 2025
School of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China. Electronic address:
Bacterial-infected wounds usually lead to slow wound healing due to increased inflammation, especially wounds infected by drug-resistant bacteria, which is a serious challenge in the biomedical field. Traditional antimicrobial strategies such as antibiotics lead to a significant increase in drug-resistant strains and have limited efficacy. Therefore, there is an urgent need to develop multifunctional dressings with excellent antibacterial activity and promotion of wound healing.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!