AI Article Synopsis

  • The study compared the effects of Abscisic Acid (ABA) on reserve accumulation in tea somatic embryos, looking specifically at starch, soluble sugars, proteins, and phenols across different developmental stages.
  • After a 14-day ABA treatment, there was a significant increase in starch and protein levels in the previously untreated embryos, along with elevated total soluble sugars.
  • The timing of ABA treatment mattered; applying it at the heart stage improved germination rates compared to untreated embryos, while treatment before or after this stage did not yield benefits.

Article Abstract

The effect of ABA on reserve accumulation in maturing somatic embryos of tea was compared with and without ABA treatment. Changes in the levels of starch, total soluble sugars (TSS), proteins, and phenols were studied in the somatic embryos at different stages of development (globular, heart, torpedo and germinating embryos) in order to investigate whether ABA could trigger accumulation of storage reserves and thereby overcome the problem of poor germination. After ABA treatment (5.0 mg l(-1)) for 14 days, the starch and protein contents that were negligible in the untreated embryos increased by several fold with a simultaneous increase in TSS. When ABA treatment occurred at the heart stage, the germination of the embryos also improved, relative to untreated controls, after ABA treatment. ABA treatment prior to or after heart stage did not improve somatic embryo germination.

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http://dx.doi.org/10.1016/j.jplph.2004.01.015DOI Listing

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